Dispersibility-Dependent Biodegradation of Graphene Oxide by Myeloperoxidase

Understanding human health risk associated with the rapidly emerging graphene‐based nanomaterials represents a great challenge because of the diversity of applications and the wide range of possible ways of exposure to this type of materials. Herein, the biodegradation of graphene oxide (GO) sheets...

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Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2015-08, Vol.11 (32), p.3985-3994
Main Authors: Kurapati, Rajendra, Russier, Julie, Squillaci, Marco A., Treossi, Emanuele, Ménard-Moyon, Cécilia, Del Rio-Castillo, Antonio Esaú, Vazquez, Ester, Samorì, Paolo, Palermo, Vincenzo, Bianco, Alberto
Format: Article
Language:English
Subjects:
Biodegradation
Biodegradation, Environmental
biopersistence
Catalysis
Chemical Sciences
Degradation
Dispersion
Graphene
graphene oxide
Graphite - chemistry
Human
Humans
Low concentrations
Medicinal Chemistry
myeloperoxidase
Nanotechnology
Oxides
Oxides - chemistry
Particle Size
Peroxidase - metabolism
safety
Spectrum Analysis, Raman
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Summary:Understanding human health risk associated with the rapidly emerging graphene‐based nanomaterials represents a great challenge because of the diversity of applications and the wide range of possible ways of exposure to this type of materials. Herein, the biodegradation of graphene oxide (GO) sheets is reported by using myeloperoxidase (hMPO) derived from human neutrophils in the presence of a low concentration of hydrogen peroxide. The degradation capability of the enzyme on three different GO samples containing different degree of oxidation on their graphenic lattice, leading to a variable dispersibility in aqueous media is compared. hMPO fails in degrading the most aggregated GO, but succeeds to completely metabolize highly dispersed GO samples. The spectroscopy and microscopy analyses provide unambiguous evidence for the key roles played by hydrophilicity, negative surface charge, and colloidal stability of the aqueous GO in their biodegradation by hMPO catalysis. The dispersibility dependent biodegradation of graphene oxide (GO) is demonstrated by using oxidative catalysis of myeloperoxidase (hMPO) derived from human neutrophils in the presence of a low concentration of hydrogen peroxide. hMPO can completely degrade the most dispersible GO but failed in degrading the least dispersed (aggregated) sample. The surface charge as well as aqueous dispersiblity of the GO samples are a playing crucial role in the biodegradation by hMPO.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201500038