An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains

• Published in: Enzyme and microbial technology 2000-06, Vol.26 (9-10), p.706-714
• Main Authors: van Dijken, J.P, Bauer, J, Brambilla, L, Duboc, P, Francois, J.M, Gancedo, C, Giuseppin, M.L.F, Heijnen, J.J, Hoare, M, Lange, H.C, Madden, E.A, Niederberger, P, Nielsen, J, Parrou, J.L, Petit, T, Porro, D, Reuss, M, van Riel, N, Rizzi, M, Steensma, H.Y, Verrips, C.T, Vindeløv, J, Pronk, J.T
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Engineering; Fundamental and applied biological sciences. Psychology; Genetics; Life Sciences; Microbiology and Parasitology; Mission oriented research; Mycology; Physiology; Physiology and metabolism; Strain choice; Yeast
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/S0141-0229(00)00162-9

To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.