Direct conjugal transfers of Ti plasmid to soil microflora

The bacterial species in soil that can receive a Ti plasmid by conjugation from Agrobacterium spp. were investigated. In order to have direct access to the potential reservoir of Ti plasmid amongst soil microflora, the conjugal system consisting of a multiply auxotrophic derivative of C58 (ST‐96‐4)...

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Bibliographic Details
Published in:Molecular ecology 1999-08, Vol.8 (8), p.1273-1284
Main Authors: Teyssier-Cuvelle, S, Mougel, C, Nesme, X
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium tumefaciens
Base Sequence
conjugation
Conjugation, Genetic
DNA Primers - chemistry
DNA, Bacterial - chemistry
Drug Resistance, Microbial - genetics
erythromycin
genbank/aj012209
genbank/aj012210
genbank/aj012211
genes
genetic markers
genetic transformation
gentamicin
kanamycin
Life Sciences
Microbial Sensitivity Tests
Molecular Sequence Data
multiple drug resistance
neomycin
nucleotide sequences
Phylogeny
plasmids
Plasmids - genetics
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
reporter genes
Rhizobiaceae
Rhizobium - classification
Rhizobium - drug effects
Rhizobium - genetics
Rhizobium rhizogenes
Rhizobium vitis
ribosomal RNA
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
rrs analysis
rrs gene
Sequence Analysis, DNA
Sinorhizobium
soil bacteria
Soil Microbiology
soil microflora
Ti plasmid
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Description
Summary:The bacterial species in soil that can receive a Ti plasmid by conjugation from Agrobacterium spp. were investigated. In order to have direct access to the potential reservoir of Ti plasmid amongst soil microflora, the conjugal system consisting of a multiply auxotrophic derivative of C58 (ST‐96‐4) and a derivative of pTiC58ΔaccR (pSTiEGK) containing a triple antibiotic‐resistance cassette in traM was used to transfer the Ti plasmid in a complex soil microflora used as the recipient. Numerous transconjugants were obtained by this method but none was identified as Agrobacterium. This could be explained by the low density of Agrobacterium in the tested soil. As indicated by analysis of the ribosomal gene rrs, transconjugants recovered directly from soil were found to be new bacterial species which appeared to be closely related to Sinorhizobium spp.
ISSN:0962-1083
1365-294X
DOI:10.1046/j.1365-294X.1999.00689.x