Genetic diversity assessed by SSR markers and chemotyping of Fusarium culmorum causal agent of foot and root rot of wheat collected from two different fields in Tunisia

Fusarium culmorum is a major pathogen able to cause foot and root rot and the incitant of Fusarium head blight in wheat in Tunisia. The aims of the present study were to evaluate by PCR the type of mycotoxins produced, to determine the mating type and to analyse the genetic diversity by microsatelli...

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Published in:European journal of plant pathology 2014-07, Vol.139 (3), p.481-495
Main Authors: Rebib, Hanene, Bouraoui, Hanene, Rouaissi, Mustapha, Brygoo, Yves, Boudabbous, Abdellatif, Hajlaoui, M. R, Sadfi-Zouaoui, Najla
Format: Article
Language:English
Subjects:
Agriculture
Biomarkers
Biomedical and Life Sciences
Causality
Ecology
Fusarium culmorum
Fusarium head blight
gene flow
genes
Genetic diversity
genetic variation
Haplotypes
intergenic DNA
Life Sciences
linkage disequilibrium
mating types
microsatellite repeats
Mycotoxins
pathogens
Plant Pathology
Plant Sciences
polymerase chain reaction
Population structure
root rot
Triticum aestivum
variance
Wheat
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Summary:Fusarium culmorum is a major pathogen able to cause foot and root rot and the incitant of Fusarium head blight in wheat in Tunisia. The aims of the present study were to evaluate by PCR the type of mycotoxins produced, to determine the mating type and to analyse the genetic diversity by microsatellite markers of 82 F. culmorum isolates recovered from two separated Tunisian fields. Specific sequences in the Tri6-Tri5 intergenic region, Tri7 and Tri13 were used to identify 3-AcDON- or 15-AcDON-. All studied F. culmorum isolates, were of the 3-AcDON- type. No 15-AcDON- and NIV types were detected in this research. Both mating types MAT1-1 and MAT1-2 were recovered from the two fields in approximately equal proportions. Five polymorphic microsatellite markers were applied to F. culmorum isolates, to determine the genetic variation in and among populations. Sixty-four haplotypes were identified; the analysis of the population structure did not reveal a strong variation between fields. Total gene diversity (H T = 0.505; H S = 0.497) and analysis of molecular variance confirmed that most of the genetic variability was within populations (Φ ST = 0.033; P 
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-014-0405-x