Genetic diversity revealed by physiological behavior of citrus genotypes subjected to salt stress

• Published in: Acta physiologiae plantarum 2015, Vol.37 (1), Article 1740
• Main Authors: Hussain, Sajjad, Morillon, Raphaël, Anjum, Muhammad Akbar, Ollitrault, Patrick, Costantino, Gilles, Luro, François
• Format: Article
• Language: English
• Subjects: Agriculture; Biomedical and Life Sciences; chlorophyll; Citroncirus webberi; Citrus medica; Environmental Sciences; females; genetic variation; genotype; hybrids; leaves; Life Sciences; mandarins; Original Paper; photosynthesis; Plant Anatomy/Development; Plant Biochemistry; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; principal component analysis; pummelos; salt stress; salt tolerance; sodium chloride; species dispersal; stomatal conductance; Vegetal Biology
• ISSN: 0137-5881; 1861-1664
• DOI: 10.1007/s11738-014-1740-4

Different physiological behavior of a wide range of varieties and species belonging to the Citrus genus was analyzed when subjected to salt stress with the aim to seek new sources of tolerance that might be specie-specific. Our goal was to use physiological results obtained along a salt stress in order to clarify if it would be possible to associate them with the known citrus genetic diversity. For that purpose, we have selected 20 different genotypes representing the major species on the basis of the genetic diversity of Citrus genus complemented with one intergeneric hybrid Carrizo citrange (C. sinensis × P. trifoliata). A moderate salt stress of 75 mM of NaCl was applied for 12 weeks. For control plants, the main parameters contributing for more than 25 % to the diversity on the two axes of principal component analysis (PCA) were chlorophyll content, photosynthesis and Fv/Fm under light. However, the dispersal of species and varieties on the PCA did not show any particular structure. Under salt stress condition, four parameters (leaf chloride content, leaf chlorophyll content, photosynthesis and stomatal conductance) contributed more specifically to the dispersion on PCA representation with more than 15 % of contribution for each parameter. Large differences were observed within citrus genus: mandarin and pummelo presented good tolerance to salt stress while citron was very sensitive. Furthermore, all secondary genotypes that presented good tolerance to salt tolerance shared mandarin or pummelo as female parent.