Caracterisation of a loss-of-function mutant of gibberellin biosynthetic gene LsGA3ox1 in lettuce

A previous study generated lettuce (Lactuca saliva) mutant lines tagged by retrotransposon Tnt1 from tobacco (Nicotiana tabacum) and identified a homozygous mutant, Tnt6a, that exhibited severe dwarf phenotype. Here we show that Tnt1 is inserted into the intron of gibberellin biosynthetic gene LsGA3...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2011, Vol.75 (12), p.2398-2400
Main Authors: Umetsu, Asami, Sawada, Yoshiaki, Mitsuhashi, Wataru, Mazier, Marianne, Toyomasu, Tomonobu
Format: Article
Language:English
Subjects:
Life Sciences
Vegetal Biology
Online Access:Get full text
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Summary:A previous study generated lettuce (Lactuca saliva) mutant lines tagged by retrotransposon Tnt1 from tobacco (Nicotiana tabacum) and identified a homozygous mutant, Tnt6a, that exhibited severe dwarf phenotype. Here we show that Tnt1 is inserted into the intron of gibberellin biosynthetic gene LsGA3ox1 in Tnt6a mutants. Expression analysis suggests that LsGA3ox1 is nearly knocked out in the Tnt6a mutants.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.110475