Consensus mapping of major resistance genes and independent QTL for quantitative resistance to sunflower downy mildew

• Published in: Theoretical and applied genetics 2012-09, Vol.125 (5), p.909-920
• Main Authors: Vincourt, Patrick, As-sadi, Falah, Bordat, Amandine, Langlade, Nicolas B, Gouzy, Jerome, Pouilly, Nicolas, Lippi, Yannick, Serre, Frédéric, Godiard, Laurence, Tourvieille de Labrouhe, Denis, Vear, Felicity
• Format: Article
• Language: English
• Subjects: Agriculture; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome Mapping; Chromosomes, Plant - genetics; Development and progression; Diseases and pests; DNA, Plant - genetics; downy mildew; Environmental Sciences; Genes; Genes, Plant - genetics; Genetic aspects; Genetic Markers; Genotype; Helianthus; Helianthus - genetics; Helianthus - immunology; Helianthus - microbiology; Helianthus annuus; Immunity, Innate - genetics; Life Sciences; Linkage Disequilibrium; major genes; Mildew; Oomycetes - pathogenicity; Original Paper; Pathogens; Physiological aspects; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant immunology; Plasmopara halstedii; Polymerase Chain Reaction; Polymorphism, Genetic - genetics; Quantitative Trait Loci; races; resistance mechanisms; root growth; stomatal movement; Sunflowers
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-012-1882-y

Major gene resistance to sunflower downy mildew (Plasmopara halstedii) races 304 and 314 was found to segregate independently from the resistance to races 334, 307 and 304 determined by the gene Pl2, already positioned on Linkage Group (LG) 8 of sunflower molecular maps. Using a consensus SSR–SNP map constructed from the INEDI RIL population and a new RIL population FU × PAZ2, the positions of Pl2 and Pl5 were confirmed and the new gene, denoted Pl21, was mapped on LG13, at 8 cM from Pl5. The two RIL populations were observed for their quantitative resistance to downy mildew in the field and both indicated the existence of a QTL on LG8 at 20–40 cM from the major resistance gene cluster. In addition, for the INEDI population, a strong QTL on LG10, reported previously, was confirmed and a third QTL was mapped on LG7. A growth chamber test methodology, significantly correlated with field results, also revealed the major QTL on LG10, explaining 65 % of variability. This QTL mapped in the same area as a gene involved in stomatal opening and root growth, which may be suggested as a possible candidate to explain the control of this character. These results indicate that it should be possible to combine major genes and other resistance mechanisms, a strategy that could help to improve durability of sunflower resistance to downy mildew.