A whole-genome radiation hybrid panel for goat

A 5000rad goat–hamster panel of 121 whole-genome radiation hybrids was generated and preliminarily characterized. A normal diploid fibroblast culture from a male Boer goat was fused with a recipient thymidine kinase-deficient hamster cell line. The generated 121 radiation hybrids were grown and prod...

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Bibliographic Details
Published in:Small ruminant research 2012-06, Vol.105 (1-3), p.114-116
Main Authors: Du, X.Y., Womack, J.E., Owens, K.E., Elliott, J.S., Sayre, B., Bottcher, P.J., Milan, D., Garcia Podesta, M., Zhao, S.H., Malek, M.
Format: Article
Language:English
Subjects:
Boer (goat breed)
CHRH5000
chromosome mapping
chromosomes
DNA
Gene mapping
genetic markers
Goat
goats
hamsters
hybrids
Life Sciences
Radiation hybrid panel
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Summary:A 5000rad goat–hamster panel of 121 whole-genome radiation hybrids was generated and preliminarily characterized. A normal diploid fibroblast culture from a male Boer goat was fused with a recipient thymidine kinase-deficient hamster cell line. The generated 121 radiation hybrids were grown and produced an average of 8.4mg of DNA per radiation hybrid. A SINE-PCR test showed that almost all radiation hybrids retained goat DNA. The retention frequencies of the 121 hybrids were preliminarily estimated using a collection of 42 unlinked molecular markers. An optimized panel of therein 90 radiation hybrids (CHRH5000) with an average retention frequency of 34.2% was screened on the basis of interspersed repetitive DNA content and chromosome retention frequency. The development of this WG-RH panel will provide a fundamental tool for advanced goat genome mapping studies and for mammalian comparative mapping.
ISSN:0921-4488
1879-0941
DOI:10.1016/j.smallrumres.2011.11.023