The Shu complex regulates Rad52 localization during rDNA repair

The Shu complex, consisting of Rad51 paralogues, is an important regulator of homologous recombination, an error-free DNA repair pathway. Consequently, when members of this complex are disrupted, cells exhibit a mutator phenotype, sensitivity to DNA damage reagents and increased gross chromosomal re...

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Bibliographic Details
Published in:DNA repair 2013-09, Vol.12 (9), p.786-790
Main Authors: Bernstein, Kara A., Juanchich, Amélie, Sunjevaric, Ivana, Rothstein, Rodney
Format: Article
Language:English
Subjects:
DNA Repair
DNA, Fungal - genetics
DNA, Fungal - metabolism
DNA, Ribosomal - genetics
DNA, Ribosomal - metabolism
Gene Knockout Techniques
Life Sciences
Nuclear Proteins - metabolism
Protein Transport
Rad52
Rad52 DNA Repair and Recombination Protein - metabolism
Rad52 sumoylation
rDNA recombination
Recombination, Genetic
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
Shu complex
Sumoylation
UAF complex
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Summary:The Shu complex, consisting of Rad51 paralogues, is an important regulator of homologous recombination, an error-free DNA repair pathway. Consequently, when members of this complex are disrupted, cells exhibit a mutator phenotype, sensitivity to DNA damage reagents and increased gross chromosomal rearrangements. Previously, we found that the Shu complex plays an important role in ribosomal DNA (rDNA) recombination when the Upstream Activating Factor (UAF) protein Uaf30 is disrupted. UAF30 encodes a protein needed for rDNA transcription and when deleted, rDNA recombination increases and the rDNA expands in a Shu1-dependent manner. Here we find using the uaf30-sensitized background that the central DNA repair protein Rad52, which is normally excluded from the nucleolus, frequently overlaps with the rDNA. This close association of Rad52 with the rDNA is dependent upon Shu1 in a uaf30 mutant. Previously, it was shown that in the absence of Rad52 sumoylation, Rad52 foci mislocalize to the nucleolus. Interestingly, here we find that using the uaf30 sensitized background the ability to regulate Rad52 sumoylation is important for Shu1 dependent rDNA recombination as well as Rad52 close association with rDNA. Our results suggest that in the absence of UAF30, the Shu complex plays a central role in Rad52 rDNA localization as long as Rad52 can be sumoylated. This discrimination is important for rDNA copy number homeostasis.
ISSN:1568-7864
1568-7856
DOI:10.1016/j.dnarep.2013.05.003