Indicators of pesticide contamination: Soil enzyme compared to functional diversity of bacterial communities via Biolog ® Ecoplates

• Published in: European journal of soil biology 2011-07, Vol.47 (4), p.256-263
• Main Authors: Floch, Carine, Chevremont, Anne-Céline, Joanico, Karine, Capowiez, Yvan, Criquet, Stéven
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Bacterial functional diversity; Biochemistry and biology; Biolog; Biological and medical sciences; Chemical, physicochemical, biochemical and biological properties; Environmental Sciences; Fundamental and applied biological sciences. Psychology; Indicator of soil contamination; Life Sciences; Microcosms; Pesticides; Physics, chemistry, biochemistry and biology of agricultural and forest soils; Soil and water pollution; Soil enzymes; Soil science
• ISSN: 1164-5563; 1778-3615
• DOI: 10.1016/j.ejsobi.2011.05.007

The aim of this study was to clarify the potential of soil enzyme activities as indicators of pesticide contamination, and to compare this to using the functional diversity of bacterial communities via Biolog ® Ecoplates. The individual effects of the pure active ingredients ( i.e. not the commercial formulations) of ten pesticides on various soil enzyme activities were recorded under laboratory conditions at different incubation times ( i.e. 0, 2, 6 and 12 months). Results clearly showed that response patterns of soil enzyme activities differed in their sensitivities to pesticide addition over time ( i.e. stimulation, inhibition or no effect). Results suggested that phenol oxidase activity could be used as an early indicator of pesticide contamination, and arylamidase and β-glucosidase could be used to evaluate soil resilience after pesticide disturbances. We concluded that the functional diversity of bacterial communities was less efficient than soil enzyme activities as an indicator of pesticide contamination. ► Pesticides and soil mesocosms. ► Soil enzymes and community-level physiological profiles (Biolog CLPP). ► Enzymes activities are more efficient than CLPP as indicator of pesticide contamination. ► Response patterns differ with enzyme type.