Impact of Processing on the Noncovalent Interactions between Procyanidin and Apple Cell Wall

• Published in: Journal of agricultural and food chemistry 2012-09, Vol.60 (37), p.9484-9494
• Main Authors: Le Bourvellec, Carine, Watrelot, Aude A, Ginies, Christian, Imberty, Anne, Renard, Catherine M. G. C
• Format: Article
• Language: English
• Subjects: Adsorption; apples; Biological and medical sciences; boiling; calorimetry; Cell Wall - chemistry; Cell Wall - metabolism; cell wall components; cell walls; Chemical and Process Engineering; cooked foods; cooking; Desiccation; drying; Engineering Sciences; Food additives; Food engineering; Food Handling - methods; Food industries; Fruit - ultrastructure; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; General aspects; Hot Temperature; Life Sciences; Malus; Microscopy, Electron, Scanning; pectins; Pectins - analysis; Plant Proteins - analysis; polymerization; Proanthocyanidins - metabolism; procyanidins; proteins; raw materials; Thermodynamics; titration
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf3015975

Procyanidins can bind cell wall material in raw product, and it could be supposed that the same mechanism of retention of procyanidins by apple cell walls takes place in cooked products. To evaluate the influence of cell wall composition and disassembly during cooking on the cell walls' capacity to interact with procyanidins, four cell wall materials differing in their protein contents and physical characteristics were prepared: cell wall with proteins, cell wall devoid of protein, and two processed cell walls differing by their drying method. Protein contents varied from 23 to 99 mg/g and surface areas from 1.26 to 3.16 m2/g. Apple procyanidins with an average polymerization degree of 8.7 were used. The adsorption of apple procyanidins on solid cell wall material was quantified using the Langmuir isotherm formulation. The protein contents in cell wall material had no effect on procyanidin/cell wall interactions, whereas modification of the cell wall material by boiling, which reduces pectin content, and drying decreased the apparent affinity and increased the apparent saturation levels when constants were expressed relative to cell wall weight. However, boiling and drying increased apparent saturation levels and had no effect on apparent affinity when the same data were expressed per surface units. Isothermal titration calorimetry indicated strong affinity (K a = 1.4 × 104 M–1) between pectins solubilized by boiling and procyanidins. This study higllights the impact of highly methylated pectins and drying, that is, composition and structure of cell wall in the cell wall/procyanidin interactions.