Plant extracts rich in polyphenols (PERP) are efficient antioxidants to prevent lipoperoxidation in plasma lipids from animals fed n − 3 PUFA supplemented diets

• Published in: Animal feed science and technology 2007-08, Vol.136 (3), p.281-296
• Main Authors: Gladine, C., Morand, C., Rock, E., Bauchart, D., Durand, D.
• Format: Article
• Language: English
• Subjects: Agricultural sciences; animal feeding; animal models; Animal production studies; antinutritional factors; Antioxidant; antioxidant activity; antioxidants; Biological and medical sciences; biomarkers; blood lipids; citrus fruits; dietary supplements; Feed and pet food industries; feed supplements; Food industries; Fundamental and applied biological sciences. Psychology; grapes; Life Sciences; Lipid; lipid peroxidation; lipids; marigold; Natural; omega-3 fatty acids; Peroxidation; plant extracts; Plasma; polyphenols; polyunsaturated fatty acids; protective effect; Rat; rats; rosemary
• ISSN: 0377-8401; 1873-2216
• DOI: 10.1016/j.anifeedsci.2006.09.003

Supplementation of domestic animals with n − 3 polyunsaturated fatty acids ( n − 3 PUFA) is becoming an accepted practice to improve the nutritional quality of lipids in animal products ( e.g., meat, milk, eggs). However, by increasing the proportion of n − 3 PUFA in plasma, this nutritional strategy also enhances its susceptibility to lipoperoxidation which has been implicated in the deterioration of many physiological functions including growth, reproduction and immunity. Dietary antioxidants are efficient means to limit lipoperoxidation in vivo and recent investigations have focused on natural molecules to satisfy consumer concerns over safety and toxicity. The aim of our study was to evaluate the in vivo antioxidant capacity of four plant extracts rich in polyphenols (PERP, i.e., rosemary, RO; grape, GP; citrus, CI or marigold, MA) in rats made highly sensitive to lipoperoxidation by feeding them a n − 3 PUFA rich diet for 3 weeks. The three following weeks, animals ( n = 60) were fed the same n − 3 PUFA rich diet without (Control) or with PERP (5 g/kg diet). At sacrifice, plasma was collected to measure the total antioxidant status (TAS), concentrations of malondiadehyde (MDA) and α-tocopherol and to evaluate kinetics of conjugated diene (CD) generation, ( i.e., lag phase, oxidation rate and maximal CD production). Intensity of lipoperoxidation (MDA production) in plasma was reduced by 12% (P