Comparative gene mapping in cattle, Indian muntjac, and Chinese muntjac by fluorescence in situ hybridization

The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n = 6 in the female and 2n = 7 in the male. The karyotypic evolution of Indian muntjac via extensive tandem fusions and several centric fusions are well documented by molecular cytogenetic studies mainly utilizing chromosome paints...

Full description

Saved in:
Bibliographic Details
Published in:Genetica 2008-11, Vol.134 (3), p.345-351
Main Authors: Murmann, Andrea E, Mincheva, Antoaneta, Scheuermann, Markus O, Gautier, Mathieu, Yang, Fentang, Buitkamp, Johannes, Strissel, Pamela L, Strick, Reiner, Rowley, Janet D, Lichter, Peter
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Biomedical and Life Sciences
Cattle
Chromosome Mapping
Chromosomes
Chromosomes, Artificial, Bacterial
Evolution, Molecular
Evolutionary Biology
Female
Fluorescence
Gene mapping
Human Genetics
Humans
Hybridization
In Situ Hybridization, Fluorescence
Karyotypes
Karyotyping
Life Sciences
Male
Microbial Genetics and Genomics
Muntjacs - classification
Muntjacs - genetics
Plant Genetics and Genomics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n = 6 in the female and 2n = 7 in the male. The karyotypic evolution of Indian muntjac via extensive tandem fusions and several centric fusions are well documented by molecular cytogenetic studies mainly utilizing chromosome paints. To achieve higher resolution mapping, a set of 42 different genomic clones coding for 37 genes and the nucleolar organizer region were used to examine homologies between the cattle (2n = 60), human (2n = 46), Indian muntjac (2n = 6/7) and Chinese muntjac (2n = 46) karyotypes. These genomic clones were mapped by fluorescence in situ hybridization (FISH). Localization of genes on all three pairs of M. m. vaginalis chromosomes and on the acrocentric chromosomes of M. reevesi allowed not only the analysis of the evolution of syntenic regions within the muntjac genus but also allowed a broader comparison of synteny with more distantly related species, such as cattle and human, to shed more light onto the evolving genome organization.
ISSN:0016-6707
1573-6857
DOI:10.1007/s10709-008-9242-1