Detection of a Specific Transposon in Erysiphe necator from Grapevines in France

The biotrophic fungus, Erysiphe necator the causal agent of the grape powdery mildew, has two genetic groups A and B in European and Australian vineyards. A strain of group A was used to isolate a DNA sequence that exhibits high sequence homology to RNaseH of a non-LTR (long tandem repeat) retrotran...

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Bibliographic Details
Published in:Journal of phytopathology 2007-06, Vol.155 (6), p.381-383
Main Authors: Bouscaut, J, Corio-C.ostet, M.-F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Erysiphe necator
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Glomerella cingulata
grape powdery mildew
group A
Life Sciences
molecular marker
Phytopathology and phytopharmacy
Phytopathology. Animal pests. Plant and forest protection
transposon
Uncinula necator
Vegetal Biology
Vitaceae
Vitis vinifera
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Summary:The biotrophic fungus, Erysiphe necator the causal agent of the grape powdery mildew, has two genetic groups A and B in European and Australian vineyards. A strain of group A was used to isolate a DNA sequence that exhibits high sequence homology to RNaseH of a non-LTR (long tandem repeat) retrotransposon of Glomerella cingulata. PCR primers were designed and tested for their specificity to genetic group A of E. necator. This molecular tool is more efficient and sensitive than nested PCR based on polymorphism in the CYP₅₁ and β-tubulin genes.
ISSN:0931-1785
1439-0434
DOI:10.1111/j.1439-0434.2007.01246.x