Genetic structure and molecular variability of Grapevine fanleaf virus populations

To gain insights into the evolutionary mechanisms of Grapevine fanleaf virus (GFLV) from the genus Nepovirus, family Secoviridae, the sequences of the complete coding region of RNA2, including genes 2A HP, 2B MP and 2C CP, and partial sequence from the RNA1-encoded gene 1E Pol of 14 GFLV isolates fr...

Full description

Saved in:
Bibliographic Details
Published in:Virus research 2010-09, Vol.152 (1), p.30-40
Main Authors: Oliver, J.E., Vigne, E., Fuchs, M.
Format: Article
Language:English
Subjects:
Arabis mosaic virus
Evolution
Genetic structure
Genetic Variation
Grapevine fanleaf virus
Life Sciences
Microbiology and Parasitology
Molecular Sequence Data
Nepovirus - classification
Nepovirus - genetics
Nepovirus - isolation & purification
Phylogenetic analysis
Phylogeny
Plant Diseases - virology
Recombination
Selection pressure
Viral Proteins - genetics
Virology
Vitis - virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To gain insights into the evolutionary mechanisms of Grapevine fanleaf virus (GFLV) from the genus Nepovirus, family Secoviridae, the sequences of the complete coding region of RNA2, including genes 2A HP, 2B MP and 2C CP, and partial sequence from the RNA1-encoded gene 1E Pol of 14 GFLV isolates from three naturally infected California vineyards were characterized. Phylogenetic analyses suggested two to three evolutionarily divergent lineages that did not reflect the vineyard origin of the isolates or an association with rootstock genotype or scion cultivar. Examination of the genetic variability of the California isolates alongside isolates worldwide, for which three RNA1 and 44 RNA2 coding sequences are available, revealed similar patterns of molecular evolution for the different regions within the GFLV genome but distinct selection constraints with the strongest pressure exerted on genes 2C CP and 2B MP, an intermediate level of pressure exerted on gene 1E Pol, and the weakest pressure exerted on gene 2A HP. Some of the California isolates resulted from interspecies recombination events between GFLV and Arabis mosaic virus with crossover sites suspected in gene 1E Pol and identified in genes 2A HP and 2B MP; and intraspecies recombination events inferred in the four target genes but most frequently observed within gene 2C CP. This study suggested that purifying selection and recombination are important evolutionary mechanisms in the genetic diversification of GFLV.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2010.05.017