In vitro effects of Tabernaemontana citrifolia extracts on Haemonchus contortus

• Published in: Research in veterinary science 2010-08, Vol.89 (1), p.88-92
• Main Authors: Marie-Magdeleine, C., Mahieu, M., D’Alexis, S., Philibert, L., Archimede, H.
• Format: Article
• Language: English
• Subjects: Adult worm motility; adult worm motility assay; Albendazole - pharmacology; Alkaloids; animal diseases; Animals; Anthelmintics - chemistry; Anthelmintics - pharmacology; Apocynaceae; bioassays; biological control; dose response; Egg hatch assay; Fruit - chemistry; Gastrointestinal nematodes; Haemonchus - drug effects; Haemonchus contortus; Hypotheses; in vitro studies; L3 migration; L3 migration inhibition assay; Larval development; larval development assay; Levamisole - pharmacology; Life Sciences; medicinal plants; Metabolites; Migration; Motility; nematode larvae; Nematodes; Parasites; plant extracts; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plant Roots - chemistry; ruminants; Tabernaemontana - chemistry; Tabernaemontana citrifolia; Veterinary medicine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2010.01.002

Tabernaemontana citrifolia ( Apocynaceae) is traditionally used as an anthelmintic preparation for ruminants in Guadeloupe (French West Indies). This study was carried out to evaluate the in vitro effect of this plant against the parasitic nematode of small ruminants Haemonchus contortus. Three extracts (aqueous, methanolic and dichloromethane) of T. citrifolia fruit, leaf and root were tested on four developmental stages of the parasite, using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition assay (LMI), and adult worm motility assay (AWM). Compared to the negative control, significant effects were observed for the different parts of T. citrifolia but with differences depending on the parasitic stage; efficacies on the larval development of H. contortus from 88.9% to 99.8% for fruit, from 72.1% to 83.8% for root and from 33.5% to 85% for leaf with dose-dependent effect for the methanolic extract. The root gave the best result on EHA (22.7% efficacy for dichloromethane extract) and AWM (56% efficacy, with dose-dependent effect for dichloromethane extract) and the leaf on LMI (49.4% efficacy). These results suggest that T. citrifolia possess anthelmintic activity against H. contortus. The active ingredients responsible for the activity could be the alkaloid compounds present in the plant parts of the plant.