Lipopolysaccharides of Pectobacterium atrosepticum and Pseudomonas corrugata Induce Different Defence Response Patterns in Tobacco, Tomato, and Potato

• Published in: Plant biology (Stuttgart, Germany) Germany), 2006-09, Vol.8 (5), p.636-645
• Main Authors: Desender, S., Klarzynski, O., Potin, P., Barzic, M.-R., Andrivon, D., Val, F.
• Format: Article
• Language: English
• Subjects: Cells, Cultured; Dose-Response Relationship, Drug; Extracellular pH; Hydrogen Peroxide - metabolism; Hydrogen-Ion Concentration; Life Sciences; lipopolysaccharide; Lipopolysaccharides - metabolism; Lipopolysaccharides - pharmacology; lipoxygenase; Lipoxygenase - metabolism; Nicotiana - drug effects; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana tabacum; Pectobacterium - metabolism; Pseudomonas - metabolism; reactive oxygen species; Research Paper; Solanaceae; Solanum lycopersicum; Solanum lycopersicum - drug effects; Solanum lycopersicum - genetics; Solanum lycopersicum - metabolism; Solanum tuberosum - drug effects; Solanum tuberosum - genetics; Solanum tuberosum - metabolism; Solanum tuberosum cvs. Bintje and Kerpondy; Species Specificity; Time Factors
• ISSN: 1435-8603; 1438-8677
• DOI: 10.1055/s-2006-924102

Abstract Lipopolysaccharides (LPS), ubiquitous cell surface components of Gram-negative bacteria, are directly implicated in plant/pathogen interactions. However, their perception by the plant, the subsequent signal transduction in both compatible and incompatible interactions, as well as the defence reactions induced in compatible interactions are as yet poorly understood. We focused on biochemical and physiological reactions induced in cell suspensions of three Solanaceae species (tobacco, tomato, and potato) by purified lipopolysaccharides from PECTOBACTERIUM ATROSEPTICUM (PA), a pathogen of potato, and PSEUDOMONAS CORRUGATA (PSC), a pathogen of tomato. LPS PA and LPS PSC caused a significant acidification of potato, tomato, and tobacco extracellular media, whereas laminarin (a linear β‐1,3 oligosaccharide elicitor) induced an alkalinisation in tobacco and tomato, but not in potato cell suspensions. None of the two LPS induced the formation of active oxygen species in any of the hosts, while laminarin induced H 2 O 2 production in cells of tobacco but not of tomato and potato. In tomato cells, LPS PA and LPS PSC induced a strong but transitory stimulation of lipoxygenase activity, whereas laminarin induced a stable or slightly increasing LOX activity over the first 24 h of contact. In tobacco, LOX activity was not triggered by either LPS, but significantly increased following treatment with laminarin. In potato, neither LPS nor laminarin induced LOX activity, in contrast with concentrated culture filtrate of PHYTOPHTHORA INFESTANS (CCF). These results demonstrate that LPS, as well as laminarin, are perceived in different ways by SOLANACEAE species, and possibly cultivars. They also suggest that defence responses modulated by LPS depend on plant genotypes rather than on the type of interaction.