Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination

Tetranychus urticae is a polyphagous mite which is an important pest of citrus worldwide. This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step...

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Bibliographic Details
Published in:Annals of applied biology 2008-10, Vol.153 (2), p.167-174
Main Authors: Hurtado, M.A, Ansaloni, T, Cros-Arteil, S, Jacas, J.A, Navajas, M
Format: Article
Language:English
Subjects:
Applications
Araneae
Biological and medical sciences
Citrus
Citrus reticulata
Fundamental and applied biological sciences. Psychology
internal transcribed spacer
internal transcribed spacers
molecular species discrimination
Panonychus citri
pest management
phylogenetic relationships
phylogeny
Prostigmata
Statistics
Tetranychidae
Tetranychus
Tetranychus spp
Tetranychus urticae
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Summary:Tetranychus urticae is a polyphagous mite which is an important pest of citrus worldwide. This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of T. urticae, species-discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of T. urticae, Tetranychus turkestani, Tetranychus evansi, Tetranychus ludeni and Panonychus citri have been determined. Results demonstrate that for these species, the rDNA ITS2 regions are much more conserved than the corresponding rDNA ITS1. The high homogeneity of the ITS2 sequence observed among the specimens of T. urticae obtained from the same ecoregion makes this DNA sequence an excellent tool for species discrimination. ITS sequences differentiate not only species but also specimens from different geographical origin. Furthermore, polymerase chain reaction-restriction fragment length polymorphism analysis of the ITS2 proved adequate for a quick screening of high numbers of field samples.
ISSN:0003-4746
1050-5164
1744-7348
2168-8737
DOI:10.1111/j.1744-7348.2008.00250.x