A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

The testis‐determining gene SRY is not well‐conserved among mammals, and particularly between mouse and other mammals. To evaluate SRY function in a nonrodent species, we produced an antibody against goat SRY and used it to investigate the expression pattern of SRY throughout goat testicular develop...

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Bibliographic Details
Published in:Developmental dynamics 2010-12, Vol.239 (12), p.3324-3335
Main Authors: Montazer‐Torbati, Fatemeh, Kocer, Ayhan, Auguste, Aurélie, Renault, Lauriane, Charpigny, Gilles, Pailhoux, Eric, Pannetier, Maëlle
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Cercopithecus aethiops
COS Cells
Development Biology
Female
Gene Expression Regulation, Developmental
germ cell
goat
Goats
Life Sciences
Male
nuclear envelope
Reverse Transcriptase Polymerase Chain Reaction
Sertoli Cells - cytology
Sertoli Cells - metabolism
sex determination
Sex Differentiation - genetics
Sex Differentiation - physiology
Sex-Determining Region Y Protein - genetics
Sex-Determining Region Y Protein - metabolism
spermatogonia
Spermatogonia - cytology
Spermatogonia - metabolism
SRY
Testis - embryology
testis differentiation
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Summary:The testis‐determining gene SRY is not well‐conserved among mammals, and particularly between mouse and other mammals. To evaluate SRY function in a nonrodent species, we produced an antibody against goat SRY and used it to investigate the expression pattern of SRY throughout goat testicular development. By contrast with the mouse, SRY is primarily expressed in most cells of XY genital‐ridges and not solely in pre‐Sertoli cells. Between cord formation and prepuberty, SRY remains expressed in both Sertoli and germinal cells. During adulthood, SRY expression declines and then disappears from meiotic germ cells, only remaining present at low levels in some spermatogonia. Unlike the germinal lineage, SRY continues to be highly expressed in adult Sertoli cells with a typical nuclear staining. Our data indicate that in goat, the role of SRY may not be limited to testis determination and could have other functions in testicular maintenance and hence male fertility. Developmental Dynamics 239:3324–3335, 2010. © 2010 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.22452