Induced parthenogenesis in mandarin for haploid production : induction procedures and genetic analysis of plantlets

This study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of 'Meyer' lemon. Pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300, 600, and 900 Gy). The resulting see...

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Bibliographic Details
Published in:Plant cell reports 2007-07, Vol.26 (7), p.937-944
Main Authors: FROELICHER, Yann, BASSENE, Jean-Baptiste, JEDIDI-NEJI, Emna, DAMBIER, Dominique, MORILLON, Raphael, BERNARDINI, Genevibve, COSTANTINO, Gilles, OLLITRAULT, Patrick
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Citrus - genetics
Citrus - growth & development
Citrus reticulata
Citrus sinensis
Dose-Response Relationship, Radiation
Eukaryotic cell cultures
Fruit - growth & development
Fruits
Fundamental and applied biological sciences. Psychology
Gamma rays
Genetics
Genotypes
Germination
Gynogenesis
Haploidy
Life Sciences
Methods. Procedures. Technologies
Miscellaneous
Parthenogenesis - genetics
Plant cells and fungal cells
Plants genetics
Pollen
Pollen - radiation effects
Pollination
Seeds - genetics
Seeds - growth & development
Seeds - radiation effects
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Summary:This study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of 'Meyer' lemon. Pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300, 600, and 900 Gy). The resulting seeds were characterised by a small size. Embryos were rescued in vitro and the ploidy level of the plantlets was determined by flow cytometry analysis. Haploid, diploid, triploid plantlets were obtained. The haploid parthenogenetic origin was confirmed using microsatellite marker analysis and chromosome count. Diploid and triploid plants were the result of crosses between mandarin and lemon. The induction of gynogenetic haploids of 'Fortune' (Citrus clementina Hort ex Tan. x Citrus tangerina Hort ex Tan.) and 'Ellendale' (Citrus reticulata Blanco x Citrus sinensis L. Osb) is reported here for the first time.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-007-0314-y