Fusarium response to oxidative stress by H₂O₂ is trichothecene chemotype-dependent

The present study aims at clarifying the impact of oxidative stress on type B trichothecene production. The responses to hydrogen peroxide (H₂O₂) of an array of Fusarium graminearum and Fusarium culmorum strains were compared, both species carrying either the chemotype deoxynivalenol (DON) or nivale...

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Bibliographic Details
Published in:FEMS microbiology letters 2009-04, Vol.293 (2), p.255-262
Main Authors: Ponts, Nadia, Couedelo, Leslie, Pinson-Gadais, Laetitia, Verdal-Bonnin, Marie-Noëlle, Barreau, Christian, Richard-Forget, Florence
Format: Article
Language:English
Subjects:
Antioxidants
Biological and medical sciences
Catalase
Catalase - metabolism
Deoxynivalenol
Fundamental and applied biological sciences. Psychology
Fungal Proteins - metabolism
Fusarium
Fusarium - drug effects
Fusarium - physiology
Fusarium culmorum
Fusarium graminearum
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
H2O2
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
H₂O
Life Sciences
Microbiology
Microbiology and Parasitology
Mycology
Nivalenol
Oxidants - pharmacology
Oxidative Stress
Stress, Physiological
Toxins
Trichothecenes - metabolism
type B trichothecenes
Up-Regulation
Virulence
Zea mays
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Summary:The present study aims at clarifying the impact of oxidative stress on type B trichothecene production. The responses to hydrogen peroxide (H₂O₂) of an array of Fusarium graminearum and Fusarium culmorum strains were compared, both species carrying either the chemotype deoxynivalenol (DON) or nivalenol (NIV). In both cases, levels of in vitro toxin production are greatly influenced by the oxidative parameters of the medium. A 0.5 mM H₂O₂ stress induces a two- to 50-fold enhancement of DON and acetyldeoxynivalenol production, whereas the same treatment results in a 2.4- to sevenfold decrease in NIV and fusarenone X accumulation. Different effects of oxidative stress on toxin production are the result of a variation in Fusarium's antioxidant defence responses according to the chemotype of the isolate. Compared with DON strains, NIV isolates have a higher H₂O₂-destroying capacity, which partially results from a significant enhancement of catalase activity induced by peroxide stress. A 0.5 mM H₂O₂ treatment leads to a 1.3- to 1.7-fold increase in the catalase activity of NIV isolates. Our data, which show the higher adaptation to oxidative stress developed by NIV isolates, are consistent with the higher virulence of these Fusarium strains on maize compared with DON isolates.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2009.01521.x