Medicago species affect the community composition of arbuscular mycorrhizal fungi associated with roots

Sequencing of the 5' end of the large ribosomal subunit (LSU rDNA) and quantitative polymerase chain reaction (qPCR) were combined to assess the impact of four annual Medicago species (Medicago laciniata, Medicago murex, Medicago polymorpha and Medicago truncatula) on the genetic diversity of a...

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Bibliographic Details
Published in:The New phytologist 2007-01, Vol.176 (1), p.197-210
Main Authors: Pivato, B, Mazurier, S, Lemanceau, P, Siblot, S, Berta, G, Mougel, C, van Tuinen, D
Format: Article
Language:English
Subjects:
Agricultural soils
annual medics
arbuscular mycorrhizal (AM) fungi
DNA Primers
DNA, Ribosomal - chemistry
Fungi
Gene Library
genetic diversity
Genetic Variation
Genotype
large ribosomal subunit (LSU rDNA)
Life Sciences
Medicago
Medicago - microbiology
Medicago polymorpha
Medicago truncatula
Mycology
Mycorrhizae - classification
Mycorrhizae - genetics
Mycorrhizae - physiology
Mycorrhizal fungi
Phylogeny
Phytopathology and phytopharmacy
Plant roots
Plant Roots - microbiology
Plants
Polymerase chain reaction
quantitative polymerase chain reaction
Ribosomal DNA
Soil fungi
Species diversity
Species Specificity
Vegetal Biology
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Summary:Sequencing of the 5' end of the large ribosomal subunit (LSU rDNA) and quantitative polymerase chain reaction (qPCR) were combined to assess the impact of four annual Medicago species (Medicago laciniata, Medicago murex, Medicago polymorpha and Medicago truncatula) on the genetic diversity of arbuscular mycorrhizal (AM) fungi, and on the relative abundance of representative AM fungal genotypes, in a silty-thin clay soil (Mas d'Imbert, France). Two hundred and forty-six Glomeromycete LSU rDNA sequences from the four plant species and the bulk soil were analysed. The high bootstrap values of the phylogenetic tree obtained allowed the delineation of 12 operational taxonomic units (OTUs), all belonging to Glomus. Specific primers targeting Glomeromycetes and major OTUs were applied to quantify their abundance by qPCR. Glomeromycetes and targeted OTUs were significantly more abundant in the root tissues than in the bulk soil, and the frequencies of three of them differed significantly in the root tissues of the different plant species. These differences indicate that, despite the absence of strict host specificity in mycorrhizal symbiosis, there was a preferential association between some AM fungal and plant genotypes.
ISSN:0028-646X
1469-8137
DOI:10.1111/j.1469-8137.2007.02151.x