Predominant Expression of Diploid Mandarin Leaf Proteome in Two Citrus Mandarin-Derived Somatic Allotetraploid Hybrids

Fusion of citrus diploid parental protoplasts generates allotetraploid hybrids which do not retain their parental traits with regard to leaf aroma compound biosynthesis. The aim of this study was thus to examine hybrid leaf proteomes in comparison with their parents. Leaf soluble proteins from two c...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2006-08, Vol.54 (17), p.6212-6218
Main Authors: Gancel, Anne-Laure, Grimplet, Jérôme, Sauvage, François-Xavier, Ollitrault, Patrick, Brillouet, Jean-Marc
Format: Article
Language:English
Subjects:
allopolyploidy
Biological and medical sciences
Citrus
Citrus - chemistry
Citrus - genetics
Citrus aurantiifolia
Citrus aurantiifolia - chemistry
Citrus aurantiifolia - genetics
Citrus deliciosa
Diploidy
Food engineering
Food industries
Fortunella margarita
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Gene Expression
Hybridization, Genetic
intergeneric hybridization
interspecific hybridization
leaves
Life Sciences
limes
plant breeding
Plant Leaves - chemistry
Plant Proteins - analysis
Plant Proteins - genetics
proteome
Proteome - analysis
Proteome - genetics
proteomics
Rutaceae - chemistry
Rutaceae - genetics
somatic hybridization
tetraploidy
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Summary:Fusion of citrus diploid parental protoplasts generates allotetraploid hybrids which do not retain their parental traits with regard to leaf aroma compound biosynthesis. The aim of this study was thus to examine hybrid leaf proteomes in comparison with their parents. Leaf soluble proteins from two citrus allotetraploid hybrids (mandarin + lime and mandarin + kumquat) and their diploid parents (mandarin, lime, and kumquat) were submitted to 2-D gel electrophoresis. Leaf proteome maps of the tetraploid hybrids were compared with those of their parents on the basis of the presence/absence of spots and of their spot relative volumes. The two allotetraploid hybrid maps were found closer to that of their mandarin parent than to those of their nonmandarin parents in terms of the presence/absence of spots as well as at a quantitative level. This approach has to be related to the already observed dominance of mandarin in allotetraploids with regard to volatile compound biosynthesis in leaves. Keywords: Tetraploid citrus somatic hybrids; Citrus deliciosa Ten.; Citrus aurantifolia (Christm.) Swing.; Fortunella margarita (Lour.) Swing.; leaf proteome 2-D electrophoresis map
ISSN:0021-8561
1520-5118
DOI:10.1021/jf060657p