Study of iron metabolism disturbances in an animal model of insulin resistance

Abstract The relationship between iron and insulin-resistance (IR) is documented by the positive correlation between iron stores and IR. Moreover, some patients exhibited a hepatic iron overload associated with IR (HIO-IR) but the mechanism involved in this overload is not known. Thus, we studied th...

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Published in:Diabetes research and clinical practice 2007-09, Vol.77 (3), p.363-370
Main Authors: Le Guenno, Guillaume, Chanséaume, Emilie, Ruivard, Marc, Morio, Béatrice, Mazur, Andrzej
Format: Article
Language:English
Subjects:
Animals
Antimicrobial Cationic Peptides - genetics
Body Weight
Diet
Disease Models, Animal
Endocrinology & Metabolism
Erythropoïesis
Gene Expression Regulation
Hemoglobins - analysis
Hepcidin
Hepcidins
High-fat/high-energy diet
Hyperglycemia
Hyperinsulinism
Insulin Resistance
IPGTT
Iron
Iron - blood
Iron - metabolism
Life Sciences
Male
Organ Size
Rats
Rats, Wistar
Transferrin - metabolism
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Summary:Abstract The relationship between iron and insulin-resistance (IR) is documented by the positive correlation between iron stores and IR. Moreover, some patients exhibited a hepatic iron overload associated with IR (HIO-IR) but the mechanism involved in this overload is not known. Thus, we studied the iron metabolism disturbances in an animal model of IR and the influence of provoked hyperglycemia/hyperinsulinemia on plasma iron parameters. Wistar rats were fed a control or a high-fat/high-energy (HF/HE) diet. Plasma glucose, insulin, iron, transferrin and transferrin saturation (TS) were measured during intra-peritoneal glucose test tolerance (IPGTT) compared to saline. Hemogram, tissue iron concentrations and hepatic hepcidin mRNA expression were determined at the end of experiment. HF/HE rats exhibited higher body and liver weights, increased IR-index and hemoglobin concentration. Iron content was lower in the spleen of HF/HE rats and tended to decrease in the liver as compared to controls. Transferrin values were higher and these of TS lower in HF/HE group. The hepcidin mRNA was 3.5-fold lower in HF/HE rats than in controls. IPGTT had no effect on iron status parameters in both groups. As reflected by higher hemoglobin concentration, IR could increase erythropoïesis which enhances iron requirement. Iron stores and TS value decreased leading to a down-regulation of hepcidin expression which increased iron absorption. Hepcidin expression should be investigated in metabolic syndrome and hepatic iron overload associated with IR.
ISSN:0168-8227
1872-8227
DOI:10.1016/j.diabres.2007.02.004