Variant isolates of human metapneumovirus subgroup B genotype 1 in Campinas, Brazil

Abstract Background Human metapneumovirus (HMPV) is a paramyxovirus associated with respiratory illness. The genotypes of HMPV isolates in Brazil have not been well characterized. Objectives To investigate the presence of HMPV in clinical samples collected from pediatric patients of two university h...

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Bibliographic Details
Published in:Journal of clinical virology 2008-05, Vol.42 (1), p.78-81
Main Authors: da Silva, Luciana Helena Antoniassi, Spilki, Fernando Rosado, Riccetto, Adriana Gut Lopes, de Almeida, Renata Servan, Baracat, Emílio Carlos Elias, Arns, Clarice Weis
Format: Article
Language:English
Subjects:
Allergy and Immunology
Base Sequence
Biological and medical sciences
Brazil
Cluster Analysis
Female
Fundamental and applied biological sciences. Psychology
Genotype
Genotype B subgroup 1
Hospitals, University
Human metapneumovirus
Human viral diseases
Humans
Infant
Infectious Disease
Infectious diseases
Life Sciences
Male
Medical sciences
Metapneumovirus - classification
Metapneumovirus - genetics
Metapneumovirus - isolation & purification
Microbiology
Miscellaneous
Molecular characterization
Molecular Sequence Data
Nasopharynx - virology
Paramyxoviridae Infections - virology
Paramyxovirus
Phylogeny
Polymorphism, Genetic
RNA, Viral - genetics
Sequence Alignment
Sequence Analysis, DNA
Viral diseases
Virology
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Summary:Abstract Background Human metapneumovirus (HMPV) is a paramyxovirus associated with respiratory illness. The genotypes of HMPV isolates in Brazil have not been well characterized. Objectives To investigate the presence of HMPV in clinical samples collected from pediatric patients of two university hospitals in the region of Campinas (São Paulo, Brazil) and to genotype them by partial sequencing of the HMPV F gene. Study design Nasopharyngeal aspirates were collected from children hospitalized between April and September, 2004 because of acute respiratory infections (ARI). Results We identified HMPV in 8 of 142 (5.6%) clinical samples. We determined through phylogenetic analysis that HMPV isolates in Campinas during the study were clustered within subgroup B genotype 1. Two of the isolates analyzed showed significant differences from previously isolated B1 viruses, when compared to HMPV isolated in South Africa and Canada, and clustered in a separate branch within this genotype. Conclusions In 2004 in our geographic region all HMPV isolates from pediatric patients were in the B1 HMPV genetic group, with two variant isolates.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2007.11.010