Successful in vitro production of embryos in the red deer ( [formula omitted][formula omitted]) and the sika deer ( [formula omitted][formula omitted])

The aim of our study was to define the conditions for IVM and IVF of oocytes in 2 common deer species as models for endangered related subspecies. Immature oocytes were recovered during the breeding season from postmortem ovaries (red deer) or by repeated laparoscopic follicular aspiration (sika dee...

Full description

Saved in:
Bibliographic Details
Published in:Theriogenology 2001-01, Vol.55 (2), p.649-659
Main Authors: Comizzoli, P., Mermillod, P., Cognié, Y., Chai, N., Legendre, X., Mauget, R.
Format: Article
Language:English
Subjects:
[formula omitted][formula omitted]
Animals
Benzimidazoles - chemistry
blastocyst
Chromatin - physiology
Coloring Agents - chemistry
Computer Science
Conservation of Natural Resources - methods
Deer - embryology
Deer - physiology
Epidermal Growth Factor - pharmacology
Female
Fertilization in Vitro - veterinary
Follicle Stimulating Hormone - pharmacology
Follicular Fluid - physiology
IVF
IVM
Life Sciences
Male
Microscopy, Fluorescence
Oocytes - physiology
ovum pickup
Pregnancy
Random Allocation
Semen Preservation - veterinary
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of our study was to define the conditions for IVM and IVF of oocytes in 2 common deer species as models for endangered related subspecies. Immature oocytes were recovered during the breeding season from postmortem ovaries (red deer) or by repeated laparoscopic follicular aspiration (sika deer). Oocytes were cultured for 24 h in IVM medium supplemented with EGF or FSH and follicular fluid. Stag semen was collected by electroejaculation (both species) or by epididymal flushing (red deer) and cryopreserved. For IVF, oocytes were exposed to different concentrations of thawed spermatozoa in a modified Tyrode albumin lactate pyruvate medium supplemented with 20% (v / v) estrus sheep serum for 18 h. After IVF, presumptive zygotes were allowed to develop in vitro for 7 days in synthetic oviduct fluid (SOF) supplemented with fetal calf serum (10%, v / v). In both species, the presence of ovine FSH and follicular fluid improved the in vitro maturation rate. In the sika deer, the optimal sperm concentration for IVF was 10 6 / mL and some fertilized oocytes reached the early morula stage (20 to 25 cells). In the red deer, after IVF with ejaculated or epididymal spermatozoa (2.0 x 10 6 / mL), 20% of zygotes developed to the blastocyst stage (50 to 80 cells).
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(01)00433-2