Efficient and specific down-regulation of prion protein expression by RNAi

Prion diseases are fatal neurodegenerative disorders associated with an abnormal isoform of the PrPc host-encoded protein. Invalidation of the Prnp gene, that encodes PrPc, led to transgenic mice that are viable, apparently healthy, and resistant to challenge by the infectious agent. These results i...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-06, Vol.305 (3), p.548-551
Main Authors: Tilly, G., Chapuis, J., Vilette, D., Laude, H., Vilotte, J.L.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Line
Down-Regulation
Life Sciences
Prions
Promoter Regions, Genetic
PrPC Proteins - genetics
PrPC Proteins - metabolism
Rabbits
RNA Interference
RNA, Messenger - metabolism
RNA, Small Interfering - chemistry
RNA, Small Interfering - genetics
RNAi
Transfected cells
Transfection
U6-vectors
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Description
Summary:Prion diseases are fatal neurodegenerative disorders associated with an abnormal isoform of the PrPc host-encoded protein. Invalidation of the Prnp gene, that encodes PrPc, led to transgenic mice that are viable, apparently healthy, and resistant to challenge by the infectious agent. These results indicated that a down-regulation of the Prnp gene expression is a potential therapeutic approach. In the present report, we demonstrate that RNAi targeted towards the Prnp mRNA can efficiently and highly specifically reduce the level of PrPc in transfected cells. It, thus, indicates that RNAi is an attractive therapeutic approach to fight against prion diseases.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(03)00805-2