Involvement of a sodium channel mutation in pyrethroid resistance in Cydia pomonella L, and development of a diagnostic test

Populations of the codling moth, Cydia pomonella L (Lepidoptera, Tortricidae) have developed resistance to several classes of insecticide such as benzoylureas, juvenile hormone analogues, ecdysone agonists and pyrethroids, but the corresponding resistance mechanisms have not been extensively studied...

Full description

Saved in:
Bibliographic Details
Published in:Pest management science 2005-06, Vol.61 (6), p.549-554
Main Authors: Brun-Barale, Alexandra, Bouvier, Jean-Charles, Pauron, David, Bergé, Jean-Baptiste, Sauphanor, Benoit
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Chemical control
Control
Cydia pomonella
Fundamental and applied biological sciences. Psychology
Insecticide resistance
Insecticide Resistance - genetics
Insecticides - pharmacology
kdr
Life Sciences
Molecular Sequence Data
Moths - genetics
Mutation
Nitriles - pharmacology
PASA
Phytopathology. Animal pests. Plant and forest protection
Point Mutation
Protozoa. Invertebrates
Pyrethrins - pharmacology
pyrethroid
Sequence Alignment
Sodium Channels - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Populations of the codling moth, Cydia pomonella L (Lepidoptera, Tortricidae) have developed resistance to several classes of insecticide such as benzoylureas, juvenile hormone analogues, ecdysone agonists and pyrethroids, but the corresponding resistance mechanisms have not been extensively studied. Knockdown resistance (kdr) to pyrethroid insecticides has been associated with point mutations in the para sodium channel gene in a great variety of insect pest species. We have studied two susceptible strains (S and Sv) and two resistant strains (Rt and Rv) of C pomonella that exhibited 4‐ and 80‐fold resistance ratios to deltamethrin, respectively. The region of the voltage‐dependent sodium channel gene which includes the position where kdr and super‐kdr mutations have been found in Musca domestica L was amplified. The kdr mutation, a leucine‐to‐phenylalanine replacement at position 1014, was found only in the Rv strain. In contrast, the super‐kdr mutation, a methionine‐to‐threonine replacement at position 918, was not detected in any C pomonella strain. These data allowed us to develop a PCR‐based diagnostic test (PASA) to monitor the frequency of the kdr mutation in natural populations of C pomonella in order to define appropriate insecticide treatments in orchards. Copyright © 2005 Society of Chemical Industry
ISSN:1526-498X
1526-4998
DOI:10.1002/ps.1002