Diversity of enterovirus sequences detected in oysters by RT-heminested PCR

• Published in: International journal of food microbiology 2004-04, Vol.92 (1), p.35-43
• Main Authors: Dubois, Eric, Merle, Ghislaine, Roquier, Catherine, Trompette, Aurélien, Le Guyader, Françoise, Crucière, Catherine, Chomel, Jean-Jacques
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cattle; Cell culture; detection; disease outbreaks; Enterovirus; Enterovirus - classification; Enterovirus - isolation & purification; Fish and seafood industries; Food Contamination; Food industries; Food Microbiology; food-borne norovirus gastroenteritis; France; Fundamental and applied biological sciences. Psychology; gastroenteritis; genetic variation; Genome, Viral; Human enterovirus; Humans; Life Sciences; Marine; messenger RNA; molecular sequence data; Norovirus; nucleotide sequences; Ostreidae - virology; oysters; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; reverse transcriptase-heminested polymerase chain reaction; RNA, Viral - analysis; RT-PCR; Sequence Alignment; Sequence analyze; Sequence Homology, Nucleic Acid; Shellfish; Shellfish - microbiology; Swine
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2003.06.001

Oysters harvested in western France, from five sites associated with outbreaks of food-borne norovirus gastroenteritis between February 2000 and March 2001, were assayed for enterovirus RNA by reverse transcriptase-heminested polymerase chain reaction (RT-heminested PCR). Forty percent (21/52) of shellfish samples (pool of seven oysters) were contaminated by enteroviruses. Infectious coxsackieviruses serotype A21 were isolated from three of these positive samples. Amplicons corresponding to 65 base sequences in the 5′ untranslated region of the enteroviral genome were analyzed by direct sequencing. Interpretable results were obtained from 18 amplicons, but mixtures of sequences confused the results from 3 samples. Sequences isolated from samples from the different sites were different but similarities were observed between sequences detected in shellfish from two sites at different dates. Sequences were also compared to sequences of human, bovine and porcine enteroviruses. Both human and animal origins of enterovirus contamination of shellfish seemed likely.