Detection of quantitative trait loci for fat androstenone levels in pigs

• Published in: Journal of animal science 2003-02, Vol.81 (2), p.385-394
• Main Authors: Quintanilla, R, Demeure, O, Bidanel, J.P, Milan, D, Iannuccelli, N, Amigues, Y, Gruand, J, Renard, C, Chevalet, C, Bonneau, M
• Format: Article
• Language: English
• Subjects: additive effect; Adipose Tissue - metabolism; age at slaughter; Alleles; Androsterone - genetics; Androsterone - metabolism; Animal genetics; Animal productions; Animals; Biological and medical sciences; cholesterol; Chromosome Mapping; Crosses, Genetic; cytochrome P-450; Fundamental and applied biological sciences. Psychology; Genetic Variation; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genotype; grandparents; Large White; Life Sciences; Likelihood Functions; loci; Male; males; Meishan; Models, Genetic; Mutation; Phenotype; phenotypic variation; Population genetics, reproduction patterns; quantitative trait loci; Quantitative Trait, Heritable; regression analysis; swine; Swine - genetics; Swine - growth & development; Swine - metabolism; Terrestrial animal productions; variance; Vertebrata; Vertebrates
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/2003.812385x

A QTL analysis of fat androstenone levels from a three-generation experimental cross between Large White and Meishan pig breeds was carried out. A total of 485 F2 males grouped in 24 full-sib families, their 29 parents and 12 grandparents were typed for 137 markers distributed over the entire porcine genome. The F2 male population was measured for fat androstenone levels at 100, 120, 140, and 160 d of age and at slaughter around 80 kg liveweight. Statistical analyses were performed using two interval mapping methods: a line-cross (LC) regression method, which assumes alternative alleles are fixed in founder lines, and a half- full-sib (HFS) maximum likelihood method, where allele substitution effects were estimated within each half- and full-sib family. Both methods revealed genomewide significant gene effects on chromosomes 3, 7, and 14. The QTL explained, respectively, 7 to 11%, 11 to 15%, and 6 to 8% of phenotypic variance. Three additional significant QTL explaining 4 to 7% of variance were detected on chromosomes 4 and 9 using LC method and on chromosome 6 using HFS method. Suggestive QTL were also obtained on chromosomes 2, 10, 11, 13, and 18. Meishan alleles were associated with higher androstenone levels, except on chromosomes 7, 10, and 13, although 10 and 13 additive effects were near zero. The QTL had essentially additive effects, except on chromosomes 4, 10, and 13. No evidence of linked QTL or imprinting effects on androstenone concentration could be found across the entire porcine genome. The steroid chromosome P450 21-hydroxylase (CYP21) and cytochrome P450 cholesterol side chain cleavage subfamily XIA (CYP11A) loci were investigated as possible candidate genes for the chromosome 7 QTL. No mutation of coding sequence has been found for CYP21. Involvement of a candidate regulatory mutation of CYP11A gene proposed by others can be excluded in our animals.