Immunolocalization of Hydrophobin HYDPt-1 from the Ectomycorrhizal Basidiomycete Pisolithus tinctorius during Colonization of Eucalyptus globulus Roots

• Published in: The New phytologist 2001-01, Vol.149 (1), p.127-135
• Main Authors: Tagu, D., De Bellis, R., Balestrini, R., De Vries, O. M. H., Piccoli, G., Stocchi, V., Bonfante, P., Martin, F.
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Antibodies; Biological and medical sciences; cell wall; Cell walls; Economic plant physiology; ectomycorrhiza; Ectomycorrhizas; Epidermal cells; Eucalyptus globulus; Fundamental and applied biological sciences. Psychology; Fungi; Gels; HYDPt-1 protein; hydrophobin; hydrophobins; Hyphae; Life Sciences; Mycelium; Parasitism and symbiosis; Phytopathology and phytopharmacy; Pisolithus tinctorius; Plant physiology and development; Proteins; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); tree; Vegetal Biology
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1046/j.1469-8137.2001.00009.x

The immunolocalization of one of the hydrophobins of Pisolithus tinctorius (HYDPt-1) is reported. Hydrophobin proteins play key roles in adhesion and aggregation of fungal hyphae, and it is already known that formation of ectomycorrhizas on eucalypt roots enhances the accumulation of hydrophobin mRNAs in the mycelium of Pisolithus tinctorius. Purification of SDS-insoluble proteins from the mycelium of P. tinctorius showed the presence of a 13 kDa polypeptide with properties of class I hydrophobin. Polyconal antibodies were raised against a recombinant HYDPt-1 polypeptide, and these were used for immunofluorescence-coupled transmission electron microscopy. HYDPt-1 is a cell wall protein located at the surface of the hyphae with no preferential accumulation in the fungal cells of the different tissues of the ectomycorrhiza (i.e. extraradical hyphae, mantle or Hartig net).