Reprogramming in nuclear transfer

Reprogramming a nucleus by transferring it to oocyte cytoplasm triggers epigenetic changes that eventually lead to the restoration of a totipotent state and the birth of a viable animal. A simplified way of studying this complex process is to study cell hybrids. These studies suggest that the plurip...

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Bibliographic Details
Published in:Current opinion in genetics & development 2003-10, Vol.13 (5), p.486-491
Main Authors: Jouneau, Alice, Renard, Jean-Paul
Format: Article
Language:English
Subjects:
Animals
Cell Nucleus - physiology
Cloning, Organism
Computer Science
Cytoplasm - physiology
Epigenesis, Genetic - physiology
Life Sciences
Mice - embryology
Nuclear Transfer Techniques
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Summary:Reprogramming a nucleus by transferring it to oocyte cytoplasm triggers epigenetic changes that eventually lead to the restoration of a totipotent state and the birth of a viable animal. A simplified way of studying this complex process is to study cell hybrids. These studies suggest that the pluripotent character of one nucleus is dominant over that of the other nucleus. The development of nuclear transfer embryos shows that there are several restriction points and that the extra-embryonic lineages may be the primary source of death. Successful reprogramming depends on the balance between epigenetic modifications and the regulative properties of development.
ISSN:0959-437X
1879-0380
DOI:10.1016/S0959-437X(03)00118-7