Cytological characterization of elicitin-induced protection in tobacco plants infected by Phytophthora parasitica or phytoplasma

• Published in: Phytopathology 2003-10, Vol.93 (10), p.1308-1319
• Main Authors: Lherminier, J, Benhamou, N, Larrue, J, Milat, M.L, Boudon-Padieu, E, Nicole, M, Blein, J.P
• Format: Article
• Language: English
• Subjects: Bacterial plant pathogens; Biological and medical sciences; calcium pectate; cell biology; cryptogein; developmental stages; disease resistance; elicitins; elicitor disease resistance; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; histochemistry; induced resistance; infection; Life Sciences; Nicotiana tabacum; oligandrin; pathogenicity; pectins; phenolic compounds; phloem; Phytopathology and phytopharmacy; Phytopathology. Animal pests. Plant and forest protection; Phytophthora nicotianae var. parasitica; Phytophthora parasitica; Phytoplasma; plant extracts; plant pathogenic fungi; plant proteins; Pythium; Vegetal Biology
• ISSN: 0031-949X; 1943-7684; 0031-949X
• DOI: 10.1094/PHYTO.2003.93.10.1308

ABSTRACT Elicitins, small proteins secreted by Phytophthora and Pythium spp., display the ability to induce plant resistance toward pathogens. Ultrastructural investigations of cryptogein-treated tobacco plants evidenced host defense responses such as (i) formation of a calcium pectate gel in intercellular spaces of parenchymas, (ii) impregnation of pectin by phenolic compounds in intercellular spaces of phloem bundles, and (iii) accumulation of phloem proteins (P proteins) in the lumen of leaf sieve elements. These cytological modifications lead to the enhancement of physical barriers that prevent pathogen ingress and restrict host tissue colonization when cryptogein-treated tobacco plants were challenged with the pathogen Phytophthora parasitica. Wall appositions also were observed at most sites of penetration of hyphae. Moreover, growing hyphae exhibited severe morphological damages, suggesting a modified toxic environment. The same induction of P proteins in mature sieve tubes of tobacco leaves was obtained with oligandrin treatment, another elicitin. Cryptogein or oligandrin treatment prevented symptom expression in phytoplasma-infected tobacco plants in contrast with nontreated tobacco plants. Moreover, P protein plugs and occlusion of pore sites by callose were evidenced in sieve elements of treated plants. Both these phloem modifications might prevent the in planta movement of phloem-restricted microorganisms.