Generation of fertile cloned rats by regulating oocyte activation

In this study, we achieved a breakthrough in cloning rats using somatic cell nuclear transfer (SCNT), overcoming longstanding challenges associated with the rapid and incomplete activation of rat oocytes following their removal from the oviduct. By employing the protease inhibitor MG132, we were abl...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2003, Vol.302
Main Authors: Qi Zhou, -, Renard, Jean Paul J. P., Le Friec, G., Brochard, Vincent V., Beaujean, Nathalie, Cherifi, Y., Fraichard, A., Cozzi, J.
Format: Article
Language:English
Subjects:
Computer Science
Life Sciences
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, we achieved a breakthrough in cloning rats using somatic cell nuclear transfer (SCNT), overcoming longstanding challenges associated with the rapid and incomplete activation of rat oocytes following their removal from the oviduct. By employing the protease inhibitor MG132, we were able to stabilize oocytes and prevent premature activation, allowing sufficient time for SCNT to be successfully performed.This refined protocol resulted in the production of viable embryos, which, when implanted into foster mothers, led to the birth of live cloned rats. These clones included individuals that reached maturity and produced healthy offspring, demonstrating the technique’s robustness. Our work establishes a foundation for precise genetic modifications in rats, such as conditional knockouts and gene replacements, making it possible to develop more relevant models for studying human diseases.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1088313