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Feruloyl esterase from Aspergillus niger: a comparison of the production in solid state and submerged fermentation

Solid state fermentation (SSF) culture conditions were investigated for the production of feruloyl esterase by Aspergillus niger I-1472 and compared with submerged culture conditions. Sugar beet pulp was tested for its ability to be used both as solid support and/or carbon substrate. Mycelial growth...

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Bibliographic Details
Published in:Process biochemistry (1991) 2002-12, Vol.38 (5), p.685-691
Main Authors: Asther, Michèle, Haon, Mireille, Roussos, Sevastianos, Record, Eric, Delattre, Michel, Lesage-Meessen, Laurence, Labat, Marc, Asther, Marcel
Format: Article
Language:English
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Summary:Solid state fermentation (SSF) culture conditions were investigated for the production of feruloyl esterase by Aspergillus niger I-1472 and compared with submerged culture conditions. Sugar beet pulp was tested for its ability to be used both as solid support and/or carbon substrate. Mycelial growth was monitored through ergosterol measurements. Under submerged culture conditions, A. niger I-1472 produced esterase active on methyl ester of cinnamic acids, principally methyl ferulate and methyl sinapinate. Under SSF culture conditions, the enzymic profile was different since significant esterase activities using methyl caffeate and methyl p-coumarate as substrate were detected, showing the presence of at least two different proteins. Northern blot analysis of the mycelium clearly indicated the expression of feruloyl esterase gene in both conditions.
ISSN:1359-5113
1873-3298
DOI:10.1016/S0032-9592(02)00196-6