The plant multigenic family of thiol peroxidases

• Published in: Free radical biology & medicine 2005-06, Vol.38 (11), p.1413-1421
• Main Authors: Rouhier, Nicolas, Jacquot, Jean-Pierre
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Ascorbate Peroxidases; Conserved Sequence; Cysteine - chemistry; Gene Expression Regulation, Plant; Genes, Plant; Genome, Plant; Glutaredoxin; Glutaredoxins; Glutathione - metabolism; Glutathione Peroxidase - metabolism; Glutathione peroxidases; Hydrogen Peroxide - metabolism; Hydroperoxides; Life Sciences; Multigene Family; Oxidoreductases - metabolism; Peroxidases - genetics; Peroxidases - metabolism; Peroxidases - physiology; Peroxiredoxins; Photosynthesis; Phylogeny; Plant; Plant Physiological Phenomena; Plant Proteins - genetics; Reactive Nitrogen Species; Reactive Oxygen Species; Thiols; Thioredoxin-Disulfide Reductase - metabolism; Thioredoxins; Thioredoxins - chemistry; Thioredoxins - metabolism
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2004.07.037

Thiol peroxidases are ubiquitous recently characterized heme-free peroxidases, which catalyze the reduction of peroxynitrites and of various peroxides by catalytic cysteine residues and thiol-containing proteins as reductants. In plants, five different classes can be distinguished, according to the number and the position of conserved catalytic cysteines. Four classes are defined as peroxiredoxins and were already identified by phylogenetic sequence analysis, 1-Cys, 2-Cys, type II, and type Q peroxiredoxins, and the fifth is represented by glutathione peroxidases, which were recently shown to possess a thioredoxin-dependent activity in plants. Since the discovery of peroxiredoxins in plants in 1996, a lot of work has been devoted to the biochemical and functional characterization of the different peroxiredoxin isoforms, but in contrast, few structural data are available. The analysis of the Arabidopsis thaliana genome indicates that at least 17 isoforms of thioredoxin-dependent peroxidases are expressed in various plant compartments. The role of these proteins is discussed in terms of electron donor and substrate specificities and in light of their expression and localization. These enzymes are expressed in many plant tissues and are involved notably in the protection of the photosynthetic apparatus, in the response to various biotic or abiotic stresses by fighting reactive oxygen or nitrogen species and lipid peroxidation.