Characterization of Markers to Determine the Extent and Variability of Leaf Senescence in Arabidopsis. A Metabolic Profiling Approach

• Published in: Plant physiology (Bethesda) 2005-06, Vol.138 (2), p.898-908
• Main Authors: Diaz, Céline, Purdy, Sarah, Christ, Aurélie, Morot-Gaudry, Jean-Francois, Wingler, Astrid, Masclaux-Daubresse, Céline
• Format: Article
• Language: English
• Subjects: Amino acids; Amino Acids - metabolism; anions; Arabidopsis - metabolism; Arabidopsis - physiology; Arabidopsis thaliana; Biological and medical sciences; biomarkers; Biomarkers - metabolism; carbohydrate content; Carbon - metabolism; Cellular senescence; Chlorophyll - metabolism; Chlorophylls; Circles; Crosses, Genetic; Environmental Stress and Adaptation; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Genetics; glucose; leaf development; leaf senescence; Leaves; Life Sciences; Metabolism; metabolome; metabolomics; Molecular Sequence Data; Nitrogen; Nitrogen - metabolism; Phenotype; plant biochemistry; Plant Leaves - metabolism; Plant Leaves - physiology; Plant physiology and development; Plants; Plants genetics; Senescence and abscission; Sugars; Sulfates; Vegetative apparatus, growth and morphogenesis. Senescence
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.105.060764

Comparison of the extent of leaf senescence depending on the genetic background of different recombinant inbred lines (RILs) of Arabidopsis (Arabidopsis thaliana) is described. Five RILs of the Bay-0 x Shahdara population showing differential leaf senescence phenotypes (from early senescing to late senescing) were selected to determine metabolic markers to discriminate Arabidopsis lines on the basis of senescence-dependent changes in metabolism. The proportion of [gamma]-aminobutyric acid, leucine, isoleucine, aspartate, and glutamate correlated with (1) the age and (2) the senescence phenotype of the RILs. Differences were observed in the glycine/serine ratio even before any senescence symptoms could be detected in the rosettes. This could be used as predictive indicator for plant senescence behavior. Surprisingly, late-senescing lines appeared to mobilize glutamine, asparagine, and sulfate more efficiently than early-senescing lines. The physiological basis of the relationship between leaf senescence and flowering time was analyzed.