Quantitative Detection of Listeria monocytogenes in Biofilms by Real-Time PCR

• Published in: Applied and Environmental Microbiology 2005-04, Vol.71 (4), p.2190-2194
• Main Authors: Guilbaud, Morgan, de Coppet, Pierre, Bourion, Fabrice, Rachman, Cinta, Prévost, Hervé, Dousset, Xavier
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Toxins - genetics; Bacteriological methods and techniques used in bacteriology; Bacteriology; Benzothiazoles; biofilm; Biofilms; Biofilms - growth & development; Biological and medical sciences; Colony Count, Microbial; Diamines; DNA Primers; DNA, Bacterial - analysis; DNA, Bacterial - isolation & purification; Ecology, environment; Food Microbiology; food pathogens; Fundamental and applied biological sciences. Psychology; genes; Heat-Shock Proteins - genetics; Hemolysin Proteins; hemolysins; Life Sciences; Listeria monocytogenes; Listeria monocytogenes - genetics; Listeria monocytogenes - growth & development; Listeria monocytogenes - isolation & purification; listeriolysin; microbial detection; Microbiology; Organic Chemicals - metabolism; polymerase chain reaction; Polymerase Chain Reaction - methods; quantitative analysis; Quantitative genetics; Quinolines; real-time polymerase chain reaction; Sensitivity and Specificity
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.71.4.2190-2194.2005

A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10² CFU/cm².