Characterization of Potato virus Y (PVY) molecular determinants involved in the vein necrosis symptom induced by PVYN isolates in infected Nicotiana tabacum cv. Xanthi

Viral molecular determinant(s) involved in the tobacco vein necrosis (TVN) symptom induced by necrotic isolates of Potato virus Y (PVY) on Nicotiana tabacum cv. Xanthi leaves remain undetermined. Reference isolates belonging to PVY(N) (infectious PVY(N)-605 clone) and PVY(O) (PVY(O)-139) were used t...

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Bibliographic Details
Published in:Journal of general virology 2005-07, Vol.86 (7), p.2101-2105
Main Authors: Tribodet, M, Glais, L, Kerlan, C, Jacquot, E
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biological and medical sciences
Cysteine Endopeptidases - chemistry
Cysteine Endopeptidases - genetics
Cysteine Endopeptidases - metabolism
Fundamental and applied biological sciences. Psychology
genes
leaves
Life Sciences
Microbiology
Microbiology and Parasitology
Miscellaneous
Molecular Sequence Data
Mutagenesis, Site-Directed
necrosis
Nicotiana - virology
Nicotiana tabacum
Plant Diseases - virology
plant diseases and disorders
Plant Leaves - virology
plant veins
Potato virus Y
Potyvirus - genetics
Potyvirus - pathogenicity
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Sequence Alignment
symptoms
tobacco
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - metabolism
Virology
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Summary:Viral molecular determinant(s) involved in the tobacco vein necrosis (TVN) symptom induced by necrotic isolates of Potato virus Y (PVY) on Nicotiana tabacum cv. Xanthi leaves remain undetermined. Reference isolates belonging to PVY(N) (infectious PVY(N)-605 clone) and PVY(O) (PVY(O)-139) were used to produce PVY chimeric genomes by using reverse-genetic techniques. These chimeric clones were inoculated biolistically onto Nicotiana clevelandii plants to establish the clone, prior to being tested on N. tabacum for their ability to induce TVN symptoms. Comparison between sequence data and symptoms observed for each mutated PVY construct shows that the C-terminal part of the multifunctional HC-Pro protein includes two residues (K400 and E419) that are involved in TVN induced by PVY(N) isolates. Site-directed mutagenesis was used to confirm that these two HC-Pro residues are involved in the TVN phenotype.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.80926-0