Vaccenic acid metabolism in the liver of rat and bovine

Hepatic metabolism of vaccenic acid (VA), especially its conversion into CLA, was studied in the bovine (ruminant species that synthesizes CLA) and in the rat (model for nonruminant) by using the in vitro technique of liver explants. Liver tissue samples were collected from fed animals (5 male Wista...

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Published in:Lipids 2005-03, Vol.40 (3), p.295-301
Main Authors: Gruffat, D, Torre, A. de la, Chardigny, J.M, Durand, D, Loreau, O, Bauchart, D
Format: Article
Language:English
Subjects:
Animal species
Animals
Biochemistry
Biochemistry, Molecular Biology
Biological Transport
Carbon dioxide
Cattle
conjugated linoleic acid
esterification
Esters - metabolism
Hepatocytes - metabolism
In Vitro Techniques
Life Sciences
lipid metabolism
Liver
Liver - metabolism
nutrient uptake
Oleic Acids - metabolism
oxidation
Oxidation-Reduction
Rats
vaccenic acid
very low density lipoprotein
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description Hepatic metabolism of vaccenic acid (VA), especially its conversion into CLA, was studied in the bovine (ruminant species that synthesizes CLA) and in the rat (model for nonruminant) by using the in vitro technique of liver explants. Liver tissue samples were collected from fed animals (5 male Wistar rats and 5 Charolais steers) and incubated at 37°C for 17 h under an atmosphere of 95% O₂/5% CO₂ in medium supplemented with 0.75 mM of FA mixture and with 55 μM [1-¹⁴C]VA. VA uptake was about sixfold lower in bovine than in rat liver slices (P
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Liver tissue samples were collected from fed animals (5 male Wistar rats and 5 Charolais steers) and incubated at 37°C for 17 h under an atmosphere of 95% O₂/5% CO₂ in medium supplemented with 0.75 mM of FA mixture and with 55 μM [1-¹⁴C]VA. VA uptake was about sixfold lower in bovine than in rat liver slices (P&lt;0.01). For both species, VA that was oxidized to partial oxidation products represented about 20% of VA incorporated by cells. The chemical structure of VA was not modified in bovine liver cells, whereas in rat liver cells, 3.2% of VA was converted into 16:0 and only 0.33% into CLA. The extent of esterification of VA was similar for both animal species (70-80% of incorporated VA). Secretion of VA as part of VLDL particles was very low and similar in rat and bovine liver (around 0.07% of incorporated VA). In conclusion, characteristics of the hepatic metabolism of VA were similar for rat and bovine animals, the liver not being involved in tissue VA conversion into CLA in spite of its high capacity for FA desaturation especially in the rat. 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Liver tissue samples were collected from fed animals (5 male Wistar rats and 5 Charolais steers) and incubated at 37°C for 17 h under an atmosphere of 95% O₂/5% CO₂ in medium supplemented with 0.75 mM of FA mixture and with 55 μM [1-¹⁴C]VA. VA uptake was about sixfold lower in bovine than in rat liver slices (P&lt;0.01). For both species, VA that was oxidized to partial oxidation products represented about 20% of VA incorporated by cells. The chemical structure of VA was not modified in bovine liver cells, whereas in rat liver cells, 3.2% of VA was converted into 16:0 and only 0.33% into CLA. The extent of esterification of VA was similar for both animal species (70-80% of incorporated VA). Secretion of VA as part of VLDL particles was very low and similar in rat and bovine liver (around 0.07% of incorporated VA). In conclusion, characteristics of the hepatic metabolism of VA were similar for rat and bovine animals, the liver not being involved in tissue VA conversion into CLA in spite of its high capacity for FA desaturation especially in the rat. This indicates that endogenous synthesis of CLA should take place exclusively in peripheral tissues.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer‐Verlag</pub><pmid>15957256</pmid><doi>10.1007/s11745-005-1385-1</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-6628-5414</orcidid><orcidid>https://orcid.org/0000-0001-8381-8999</orcidid><orcidid>https://orcid.org/0000-0002-5768-4066</orcidid></addata></record>
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subjects Animal species
Animals
Biochemistry
Biochemistry, Molecular Biology
Biological Transport
Carbon dioxide
Cattle
conjugated linoleic acid
esterification
Esters - metabolism
Hepatocytes - metabolism
In Vitro Techniques
Life Sciences
lipid metabolism
Liver
Liver - metabolism
nutrient uptake
Oleic Acids - metabolism
oxidation
Oxidation-Reduction
Rats
vaccenic acid
very low density lipoprotein
title Vaccenic acid metabolism in the liver of rat and bovine
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