Evaluation of Monocot and Eudicot Divergence Using the Sugarcane Transcriptome

• Published in: Plant physiology (Bethesda) 2004-03, Vol.134 (3), p.951-959
• Main Authors: Vincentz, Michel, Frank A. A. Cara, Okura, Vagner K., Felipe R. da Silva, Guilherme L. Pedrosa, Hemerly, Adriana S., Adriana N. Capella, Marins, Mozart, Ferreira, Paulo C., Suzelei C. França, Laurent Grivet, Vettore, Andre L., Kemper, Edson L., Willian L. Burnquist, Maria L. P. Targon, Walter J. Siqueira, Eiko E. Kuramae, Celso L. Marino, Luis E. A. Camargo, Carrer, Helaine, Luis L. Coutinho, Luiz R. Furlan, Manoel V. F. Lemos, Luiz R. Nunes, Suely L. Gomes, Santelli, Roberto V., Maria H. Goldman, Maurício Bacci Jr, Eder A. Giglioti, Thiemann, Otávio H., Flávio H. Silva, Marie-Anne Van Sluys, Nobrega, Francisco G., Arruda, Paulo, Carlos F. M. Menck
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Angiosperms; Arabidopsis; Arabidopsis - classification; Arabidopsis - genetics; Biological and medical sciences; Biotechnology; Chromosomes, Plant - genetics; Consensus Sequence; Evolution; Evolution, Molecular; Evolutionary genetics; Expressed Sequence Tags; Fundamental and applied biological sciences. Psychology; Genes; Genetic engineering; Genetic engineering applications; Genetic technics; Genetics; Genetics and breeding of economic plants; Genome Analysis; Genome, Plant; Genomes; Life Sciences; Magnoliopsida - classification; Magnoliopsida - genetics; Methods. Procedures. Technologies; Oryza - classification; Oryza - genetics; Oryza sativa; Plant breeding: fundamental aspects and methodology; Plants; Plants genetics; Qualitative comparative analysis; Rice; Saccharum - classification; Saccharum - genetics; Saccharum officinarum; Sugar cane; Transcription, Genetic; Transcriptomes; Transgenic animals and transgenic plants; Transgenic plants
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.103.033878

Over 40,000 sugarcane (Saccharum officinarum) consensus sequences assembled from 237,954 expressed sequence tags were compared with the protein and DNA sequences from other angiosperms, including the genomes of Arabidopsis and rice (Oryza sativa). Approximately two-thirds of the sugarcane transcriptome have similar sequences in Arabidopsis. These sequences may represent a core set of proteins or protein domains that are conserved among monocots and eudicots and probably encode for essential angiosperm functions. The remaining sequences represent putative monocot-specific genetic material, one-half of which were found only in sugarcane. These monocot-specific cDNAs represent either novelties or, in many cases, fast-evolving sequences that diverged substantially from their eudicot homologs. The wide comparative genome analysis presented here provides information on the evolutionary changes that underlie the divergence of monocots and eudicots. Our comparative analysis also led to the identification of several not yet annotated putative genes and possible gene loss events in Arabidopsis.