Isolation and characterization of microsatellite loci from the entomopathogenic hyphomycete, Paecilomyces fumosoroseus

Nine microsatellite markers were isolated from the entomopathogenic haploid fungus Paecilomyces fumosoroseus. Genetic diversity was assessed in 26 P. fumosoroseus isolates originated from the whitefly Bemisia tabaci collected in various geoclimatic areas. Eight loci were polymorphic with an observed...

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Bibliographic Details
Published in:Molecular ecology notes 2005-09, Vol.5 (3), p.496-498
Main Authors: Dalleau-Clouet, C, Gauthier, N, Risterucci, A.M, Bon, M.C, Fargues, J
Format: Article
Language:English
Subjects:
alleles
alleles per locus
Bemisia tabaci
DNA primers
Ecology, environment
entomopathogenic fungi
genetic diversity
genetic markers
genetic polymorphism
genetic variation
host insect
hyphomycetes
isolation
Life Sciences
loci
microsatellite repeats
microsatellites
molecular sequence data
nucleotide sequences
Paecilomyces fumosoroseus
polymerase chain reaction
polymorphic loci
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Summary:Nine microsatellite markers were isolated from the entomopathogenic haploid fungus Paecilomyces fumosoroseus. Genetic diversity was assessed in 26 P. fumosoroseus isolates originated from the whitefly Bemisia tabaci collected in various geoclimatic areas. Eight loci were polymorphic with an observed number of alleles ranging from two to six. The loci differentiated some isolates and group of isolates according to their geographical location, showing promise for the study of gene flow. All loci failed to give clear amplifications in P. fumosoroseus isolates from hosts other than B. tabaci. These microsatellite markers provide powerful tools for ecological, epidemiological, and population genetic studies.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00968.x