Plant CDC2 is not only targeted to the pre-prophase band, but also co-localizes with the spindle, phragmoplast, and chromosomes

• Published in: FEBS letters 1997-12, Vol.418 (3), p.229-234
• Main Authors: Stals, Hilde, Bauwens, Serge, Traas, Jan, Van Montagu, Marc, Engler, Gilbert, Inzé, Dirk
• Format: Article
• Language: English
• Subjects: 4′,6-diamidino-2-phenylindole; Arabidopsis - cytology; Arabidopsis - enzymology; Arabidopsis thaliana; bovine serum albumin; BSA; CDC; CDC2 Protein Kinase - metabolism; CDK; Cell Cycle; cell division cycle; Cellular Biology; Chromosomes; CLSM; confocal laser scanning microscopy; cyclin-dependent kinase; DAPI; EGTA; ethylene glycol-bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic acid; Immunolocalization; Life Sciences; Medicago sativa; microtubule-associated protein; microtubule-stabilizing buffer; Mitosis; MTSB; p34 cdc2 kinase; p34cdc2 kinase; piperazine-N,N′-bis(ethanesulfonic acid); PIPES; Plant Proteins - metabolism; polyoxyethylenesorbitan; PPB; pre-prophase band; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; soybean trypsin inhibitor; STBI; Tween
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(97)01368-9

A polyclonal antiserum against the p34 cdc2 homologue of Arabidopsis thaliana, CDC2aAt, was used in parallel with a polyclonal antiserum against the PSTAIRE motif to study the subcellular localization of CDC2 during the cell cycle of isolated root tip cells of Medicago sativa. During interphase, CDC2 was located in the nucleus and in the cytoplasm. The cytoplasmic localization persisted during the complete cell cycle, whereas the nuclear signal disappeared at nuclear envelope breakdown. At the beginning of anaphase, the anti-CDC2aAt antibody transiently co-localized with condensed chromosomes. The chromosomal co-localization disappeared as anaphase continued and remained excluded from the separated chromosomes until cytokinesis, when CDC2 re-located to the newly forming nuclei. We also observed a co-localization of CDC2 with three microtubular structures, the pre-prophase band, the spindle, and the phragmoplast.