Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region

A rapid and reliable PCR-based method for distinguishing closely related species within two groups of lactobacilli is described. Primers complementary to species-specific sequences in the 16S/23S rDNA spacer regions were designed after sequencing and sequence comparison of the spacer regions of 32 s...

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Bibliographic Details
Published in:FEMS microbiology letters 1998-04, Vol.161 (1), p.97-106
Main Authors: Berthier, F, Ehrlich, S.D
Format: Article
Language:English
Subjects:
16S/23S rRNA spacer region
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
Biological and medical sciences
Biotechnology
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
genbank/u97135
genbank/u97136
genbank/u97137
genbank/u97138
genbank/u97139
genbank/u97141
Hybridization
L. curvatus
L. graminis
L. paraplantarum
L. pentosus
L. plantarum
L. sake
Lactobacilli
Lactobacillus
Lactobacillus - classification
Life Sciences
Microbiology
Microbiology and Parasitology
Molecular Sequence Data
Polymerase Chain Reaction
Primers
ribosomal RNA
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 23S - genetics
rRNA 16S
rRNA 23S
Sensitivity and Specificity
sequence alignment
Spacer region
Species
Species‐specific probe
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Description
Summary:A rapid and reliable PCR-based method for distinguishing closely related species within two groups of lactobacilli is described. Primers complementary to species-specific sequences in the 16S/23S rDNA spacer regions were designed after sequencing and sequence comparison of the spacer regions of 32 strains. The strains belong to two groups of closely related Lactobacillus species; one composed of Lactbacillus curvatus, Lactobacillus graminis and Lactobacillus sake, the other of Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus plantarum. PCR assays with the designed primers and subsequent agarose gel analysis of the amplified fragments allowed the same species identification as the DNA/DNA hybridization procedure.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1998.tb12934.x