Expression of the growth hormone gene in ovine placenta: detection and cellular localization of the protein

In several species, placenta has been found to express GH-related proteins. In the ovine placenta, such a protein, ovine chorionic somatommamotropin, has been described, but its involvement in the fetal/placental growth process is not clearly established. The aim of this study was to investigate the...

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Bibliographic Details
Published in:Endocrinology (Philadelphia) 1996-11, Vol.137 (11), p.4886-4892
Main Authors: Lacroix, M C, Devinoy, E, Servely, J L, Puissant, C, Kann, G
Format: Article
Language:English
Subjects:
Amino acids
Animals
Base Sequence
Blotting, Northern
Cloning, Molecular
DNA Primers
Endocrinology and metabolism
Female
Fetuses
Gene expression
Growth Hormone - analysis
Growth Hormone - biosynthesis
Growth hormones
Human health and pathology
Immunoreactivity
Life Sciences
Localization
Molecular Sequence Data
Nucleotide sequence
Pituitary
Placenta
Placenta - cytology
Placenta - metabolism
Polymerase Chain Reaction
Pregnancy
Proteins
Recombinant Proteins - biosynthesis
Ribonucleic acid
RNA
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Sequence analysis
Sheep
Time Factors
Transcription, Genetic
Trophectoderm
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Summary:In several species, placenta has been found to express GH-related proteins. In the ovine placenta, such a protein, ovine chorionic somatommamotropin, has been described, but its involvement in the fetal/placental growth process is not clearly established. The aim of this study was to investigate the occurrence of another GH-related peptide in the ovine placenta. Placental extracts (days 30-140 of pregnancy) showed GH immunoreactivity between days 35-70. SDS-PAGE analysis of these extracts indicated that this immunoreactivity corresponded to 22- and 28-kDa proteins. GH-like immunoreactivity was localized on cotyledonary frozen sections in the syncytium and the trophectoderm. Northern blot analysis of placental RNA showed the expression of GH-hybridizing transcripts migrating to the same position as that of GH pituitary messenger RNA (mRNA). Those transcripts were highly expressed between days 40 and 50. Their sequence analysis showed the existence of three GH mRNA (GHP1, GHP2, and GHP3). GHP1 is identical to pituitary GH mRNA and probably codes for the 22-kDa protein. GHP2 and GHP3 encode the same protein, which differs from GHP1 by four amino acids. This study establishes the expression of GH gene and GH-immunoreactive proteins in the ovine placenta.
ISSN:0013-7227
1945-7170
DOI:10.1210/en.137.11.4886