Studies on ion channel antagonist-binding sites in sunflower protoplasts

The cytological location of ion channel antagonist-binding sites was studied in sunflower protoplasts using the fluorescent probes DM–Bodipy–PAA and DM–Bodipy–DHP. The binding specificity of the probes was established by competition experiments with Bepridil, phenylalkylamine (Verapamil) and dihydro...

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Bibliographic Details
Published in:FEBS letters 1997-07, Vol.411 (1), p.115-118
Main Authors: Vallée, N., Brière, C., Petitprez, M., Barthou, H., Souvré, A., Alibert, G.
Format: Article
Language:English
Subjects:
Binding Sites
Boron Compounds
Calcium Channel Blockers - metabolism
Cellular Biology
Dihydropyridine
Dimethyl Sulfoxide
Fluorescent Dyes - metabolism
Helianthus - metabolism
Ion channel
Ion Channels - antagonists & inhibitors
Life Sciences
Microscopy, Confocal
Microscopy, Fluorescence
Nifedipine - metabolism
Phenylalkylamine
Protoplasts
Spectrometry, Fluorescence
Sunflower protoplast
Verapamil - metabolism
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Summary:The cytological location of ion channel antagonist-binding sites was studied in sunflower protoplasts using the fluorescent probes DM–Bodipy–PAA and DM–Bodipy–DHP. The binding specificity of the probes was established by competition experiments with Bepridil, phenylalkylamine (Verapamil) and dihydropyridine (Nifedipine) which are known as calcium and potassium channel antagonists. Quantitative image analysis of the fluorescence emitted by the protoplasts showed the existence of interactions between PAA- and DHP-binding sites. Moreover, studies on the cytolocalization of the PAA receptors by confocal imaging showed that in freshly isolated protoplasts, DM–Bodipy–PAA binds exclusively at sites located in the cortical region of the cell.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)00675-3