Mapping of 14 expressed sequence tags (ESTs) from porcine skeletal muscle by somatic cell hybrid analysis

Chromosomal assignments are reported for fourteen porcine expressed sequence tags (ESTs) —CALM1, CRYAB, MYH7, MYL1, PDK4, PGAM2, PYGM, REV3L, RFC1, SLN, SPTBN1, SRM160, TPM1 and YWHAG. The ESTs were derived from our porcine skeletal muscle cDNA library. The ESTs sequences selected for mapping includ...

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Bibliographic Details
Published in:Animal genetics 2000-12, Vol.31 (6), p.400-403
Main Authors: Davoli, R, Bigi, D, Fontanesi, L, Zambonelli, P, Yerle, M, Zijlstra, C, Bosma, A A, Robic, A, Russo, V
Format: Article
Language:English
Subjects:
Animals
Base Sequence
CALM1 gene
comparative gene mapping
CRYAB gene
DNA Primers
Expressed Sequence Tags
Hybrid Cells
Life Sciences
Muscle, Skeletal - metabolism
MYH7 gene
MYL1 gene
PDK4 gene
PGAM2 gene
pig
PYGM gene
REV3L gene
RFC1 gene
skeletal muscle
SLN gene
somatic cell hybrid panel
SPTBN1 gene
SRM160 gene
Swine - genetics
TPM1 gene
YWHAG gene
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Summary:Chromosomal assignments are reported for fourteen porcine expressed sequence tags (ESTs) —CALM1, CRYAB, MYH7, MYL1, PDK4, PGAM2, PYGM, REV3L, RFC1, SLN, SPTBN1, SRM160, TPM1 and YWHAG. The ESTs were derived from our porcine skeletal muscle cDNA library. The ESTs sequences selected for mapping included the presence of the 3′‐untranslated region. The assignments were performed using two independent somatic cell hybrid panels providing the possibility of confirmation of the results obtained. The observed localizations are compared with the locations predicted from heterologous (human–pig, pig–human) chromosome painting data and knowledge of the map locations of the human homologues. These results add new information to the porcine genome transcript map.
ISSN:0268-9146
1365-2052
DOI:10.1046/j.1365-2052.2000.00687.x