Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI)

The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30min and at 2, 4 and 24h (T0, T30, T2...

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Bibliographic Details
Published in:Theriogenology 1998-08, Vol.50 (3), p.487-493
Main Authors: Chalah, T, Brillard, J.P
Format: Article
Language:English
Subjects:
Aniline Compounds
Animal biology
Animals
BIOLOGICAL PRESERVATION
Cell Survival
COCKS
Coloring Agents
CONGELACION
CONGELATION
CONSERVACION BIOLOGICA
CONSERVACION DEL SEMEN
CONSERVATION BIOLOGIQUE
CONSERVATION DU SPERME
COQ
CRYOPRESERVATION
DECONGELATION
DESCONGELACION
Eosine Yellowish-(YS)
ESPERMATOZOO
Fluorescence
Fluorescent Dyes
fowl
FREEZING
FROZEN SEMEN
GALLOS
Life Sciences
Male
METHODE
METHODS
METODOS
microscopy
Poultry - physiology
SEMEN
SEMEN DILUENTS
SEMEN PRESERVATION
SPERMATOZOA
Spermatozoa - physiology
SPERMATOZOIDE
SPERME
STAINING
Staining and Labeling
THAWING
Veterinary medicine and animal Health
VIABILIDAD
VIABILITE
VIABILITY
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Summary:The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30min and at 2, 4 and 24h (T0, T30, T2, T4, and T24) after in vitro storage (4°C, agitated) of fresh or frozen-thawed semen, the dual association SYBR-14/PI was more effective than eosin/nigrosin (P
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(98)00155-1