Characterization of hsr201 and hsr515, two tobacco genes preferentially expressed during the hypersensitive reaction provoked by phytopathogenic bacteria

• Published in: Plant molecular biology 1996-05, Vol.31 (2), p.255-265
• Main Authors: Czernic, P. (Laboratoire de Biologie Moleculaire des Relations Plantes-Microorganismes CNRS, Castanet-Tolosan (France)), Huang, H.C, Marco, Y
• Format: Article
• Language: English
• Subjects: ADN; Amino Acid Sequence; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Biochemistry, Molecular Biology; CITOCROMO P 450; CYTOCHROME P 450; Cytochrome P-450 Enzyme System - genetics; DISEASE RESISTANCE; DNA; DNA Primers - chemistry; EXPRESION GENICA; EXPRESSION DES GENES; GENE EXPRESSION; Gene Expression Regulation, Plant; Genes, Plant; HYPERSENSITIVITY; Life Sciences; MESSENGER RNA; Mixed Function Oxygenases - metabolism; Molecular Sequence Data; NICOTIANA; Nicotiana - genetics; Nicotiana tabacum; Oxygenases; Plant Diseases - genetics; Plant Proteins - genetics; Plants, Toxic; Pseudomonas Infections - genetics; PSEUDOMONAS SOLANACEARUM; REACCIONES ALERGICAS; REACTION ALLERGIQUE; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; RNA, Messenger - genetics; RNA, Plant - genetics; Sequence Alignment; Sequence Homology, Amino Acid
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/bf00021788

During an incompatible interaction between tobacco and the bacterial phytopathogen Pseudomonas solanacearum, 2 classes of genes, the so-called hsr (hypersensitivity-related) genes, activated preferentially during the hypersensitive reaction, and the str (sensitivity-related) genes, expressed strongly during compatible and incompatible interactions, have been identified. In this report, two hsr cDNA clones, hsr515 and hsr201, as well as their expression patterns are presented. Hsr515 was found to encode a P450 monooxygenase and is most similar to the ripening-related avocado gene CYP71A1 (40.6% amino acid identity). Hsr201 presents 58.6% amino acid identity with pTom36, a tomato gene expressed during fruit maturation. The putative functions of the hsr gene products appear to be quite diverse and their characteristics of activation were found to be very conserved: accumulation of the corresponding mRNAs primarily in leaf areas in contact with the avirulent P. solanacearum strain or with a Pseudomonas fluorescens strain containing the hrpZ gene encoding a necrotizing polypeptide, harpin and absence of expression during normal plant development. Our results also suggest that, in a tobacco line expressing NahG, a lower level of salicylic acid, a compound associated with systemic acquired resistance, and also possibly involved in the development of necrotic lesions characteristic of the HR, does not affect the hsr gene expression.