Expression of a cysteine-rich protein (CRP) encoding gene during early development of the trout

• Published in: Mechanisms of development 1998-08, Vol.76 (1), p.179-183
• Main Authors: Delalande, J.M, Rescan, P.Y
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Avian Proteins; Cysteine-rich protein; Embryo, Nonmammalian; Fish Proteins; Gene Expression Regulation, Developmental; In Situ Hybridization; Life Sciences; LIM domain; Mesoderm; Molecular Sequence Data; Muscle Proteins - genetics; Muscle Proteins - metabolism; Muscle, Skeletal - metabolism; Myogenesis; Oncorhynchus - embryology; Oncorhynchus - genetics; Proteins - genetics; Proteins - metabolism; Proto-Oncogene Proteins c-myc - genetics; Proto-Oncogene Proteins c-myc - metabolism; Sequence Homology, Amino Acid; Teleost
• ISSN: 0925-4773; 1872-6356
• DOI: 10.1016/S0925-4773(98)00127-0

Members of the cysteine-rich protein (CRP) define a subclass of LIM-only proteins implicated mainly in muscle differentiation. Until now, very little is known concerning the expression of CRP encoding genes during vertebrate development. We describe here the isolation of a trout ( Oncorhynchus mykiss) gene encoding a cysteine-rich protein (TCRP) and the pattern of its mRNA accumulation during embryogenesis, focusing on somitogenesis. TCRP encodes a putative protein with two LIM domains linked to a short glycine-rich region that displays 86%, 76%, 67% identity with chicken CRP2, CRP1 and MLP/CRP3 proteins, respectively. Whole-mount in situ hybridisation showed that TCRP transcript is first detected just before somitogenesis in the paraxial mesoderm, while it is absent in the axial structures. During somitogenesis, the expression of TCRP was observed caudally in the elongating presomitic mesoderm and in the last formed somites. The labelling for TCRP was found to fade as the somites mature. At the end of the somitogenesis, TCRP transcripts accumulation was restricted to pronephros and branchial arches.